Triple Quadrupole Versus High Resolution Quadrupole-Time-of-Flight Mass Spectrometry for Quantitative LC-MS/MS Analysis of 25-Hydroxyvitamin D in Human Serum

Overview

Journal J Am Soc Mass Spectrom

Specialty Chemistry

Date 2016 May 8

PMID 27154021

Citations 7

Authors

Timon Geib

Lekha Sleno

Rabea A Hall

Caroline S Stokes

Dietrich A Volmer

Affiliations

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Abstract

We describe a systematic comparison of high and low resolution LC-MS/MS assays for quantification of 25-hydroxyvitamin D3 in human serum. Identical sample preparation, chromatography separations, electrospray ionization sources, precursor ion selection, and ion activation were used; the two assays differed only in the implemented final mass analyzer stage; viz. high resolution quadrupole-quadrupole-time-of-flight (QqTOF) versus low resolution triple quadrupole instruments. The results were assessed against measured concentration levels from a routine clinical chemiluminescence immunoassay. Isobaric interferences prevented the simple use of TOF-MS spectra for extraction of accurate masses and necessitated the application of collision-induced dissociation on the QqTOF platform. The two mass spectrometry assays provided very similar analytical figures of merit, reflecting the lack of relevant isobaric interferences in the MS/MS domain, and were successfully applied to determine the levels of 25-hydroxyvitamin D for patients with chronic liver disease. Graphical Abstract ᅟ.

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References

Qi Y, Geib T, Schorr P, Meier F, Volmer D . On the isobaric space of 25-hydroxyvitamin D in human serum: potential for interferences in liquid chromatography/tandem mass spectrometry, systematic errors and accuracy issues. Rapid Commun Mass Spectrom. 2014; 29(1):1-9. DOI: 10.1002/rcm.7075. View

van den Ouweland J, Beijers A, van Daal H . Overestimation of 25-hydroxyvitamin D3 by increased ionisation efficiency of 3-epi-25-hydroxyvitamin D3 in LC-MS/MS methods not separating both metabolites as determined by an LC-MS/MS method for separate quantification of 25-hydroxyvitamin D3,.... J Chromatogr B Analyt Technol Biomed Life Sci. 2014; 967:195-202. DOI: 10.1016/j.jchromb.2014.07.021. View

Schleicher R, Encisco S, Chaudhary-Webb M, Paliakov E, McCoy L, Pfeiffer C . Isotope dilution ultra performance liquid chromatography-tandem mass spectrometry method for simultaneous measurement of 25-hydroxyvitamin D2, 25-hydroxyvitamin D3 and 3-epi-25-hydroxyvitamin D3 in human serum. Clin Chim Acta. 2011; 412(17-18):1594-9. DOI: 10.1016/j.cca.2011.05.010. View

Volmer D, Mendes L, Stokes C . Analysis of vitamin D metabolic markers by mass spectrometry: current techniques, limitations of the "gold standard" method, and anticipated future directions. Mass Spectrom Rev. 2013; 34(1):2-23. DOI: 10.1002/mas.21408. View

Liebisch G, Matysik S . Accurate and reliable quantification of 25-hydroxy-vitamin D species by liquid chromatography high-resolution tandem mass spectrometry. J Lipid Res. 2015; 56(6):1234-9. PMC: 4442879. DOI: 10.1194/jlr.D058511. View

Stokes C, Grunhage F, Baus C, Volmer D, Wagenpfeil S, Riemenschneider M . Vitamin D supplementation reduces depressive symptoms in patients with chronic liver disease. Clin Nutr. 2015; 35(4):950-7. DOI: 10.1016/j.clnu.2015.07.004. View

Ding S, Schoenmakers I, Jones K, Koulman A, Prentice A, Volmer D . Quantitative determination of vitamin D metabolites in plasma using UHPLC-MS/MS. Anal Bioanal Chem. 2010; 398(2):779-89. PMC: 2939348. DOI: 10.1007/s00216-010-3993-0. View

Abdel-Khalik J, Crick P, Carter G, Makin H, Wang Y, Griffiths W . Studies on the analysis of 25-hydroxyvitamin D(3) by isotope-dilution liquid chromatography-tandem mass spectrometry using enzyme-assisted derivatisation. Biochem Biophys Res Commun. 2014; 446(3):745-50. PMC: 4000436. DOI: 10.1016/j.bbrc.2014.01.088. View

Roth H, Schmidt-Gayk H, Weber H, Niederau C . Accuracy and clinical implications of seven 25-hydroxyvitamin D methods compared with liquid chromatography-tandem mass spectrometry as a reference. Ann Clin Biochem. 2008; 45(Pt 2):153-9. DOI: 10.1258/acb.2007.007091. View

10.

Meyer M, Helfer A, Maurer H . Current position of high-resolution MS for drug quantification in clinical & forensic toxicology. Bioanalysis. 2014; 6(17):2275-84. DOI: 10.4155/bio.14.164. View

11.

Couchman L, Benton C, Moniz C . Variability in the analysis of 25-hydroxyvitamin D by liquid chromatography-tandem mass spectrometry: the devil is in the detail. Clin Chim Acta. 2012; 413(15-16):1239-43. DOI: 10.1016/j.cca.2012.04.003. View

12.

Shah I, James R, Barker J, Petroczi A, Naughton D . Misleading measures in Vitamin D analysis: a novel LC-MS/MS assay to account for epimers and isobars. Nutr J. 2011; 10:46. PMC: 3114718. DOI: 10.1186/1475-2891-10-46. View

13.

Stokes C, Volmer D, Grunhage F, Lammert F . Vitamin D in chronic liver disease. Liver Int. 2013; 33(3):338-52. DOI: 10.1111/liv.12106. View

14.

Lensmeyer G, Poquette M, Wiebe D, Binkley N . The C-3 epimer of 25-hydroxyvitamin D(3) is present in adult serum. J Clin Endocrinol Metab. 2011; 97(1):163-8. DOI: 10.1210/jc.2011-0584. View

15.

Nurnberg B, Graber S, Gartner B, Geisel J, Pfohler C, Schadendorf D . Reduced serum 25-hydroxyvitamin D levels in stage IV melanoma patients. Anticancer Res. 2009; 29(9):3669-74. View

16.

Muller M, Stokes C, Lammert F, Volmer D . Chemotyping the distribution of vitamin D metabolites in human serum. Sci Rep. 2016; 6:21080. PMC: 4750036. DOI: 10.1038/srep21080. View

17.

Geib T, Meier F, Schorr P, Lammert F, Stokes C, Volmer D . A simple micro-extraction plate assay for automated LC-MS/MS analysis of human serum 25-hydroxyvitamin D levels. J Mass Spectrom. 2015; 50(1):275-9. DOI: 10.1002/jms.3522. View

18.

Carter G . 25-hydroxyvitamin D: a difficult analyte. Clin Chem. 2012; 58(3):486-8. DOI: 10.1373/clinchem.2011.180562. View

19.

Ramanathan R, Jemal M, Ramagiri S, Xia Y, Humpreys W, Olah T . It is time for a paradigm shift in drug discovery bioanalysis: from SRM to HRMS. J Mass Spectrom. 2011; 46(6):595-601. DOI: 10.1002/jms.1921. View

20.

Maunsell Z, Wright D, Rainbow S . Routine isotope-dilution liquid chromatography-tandem mass spectrometry assay for simultaneous measurement of the 25-hydroxy metabolites of vitamins D2 and D3. Clin Chem. 2005; 51(9):1683-90. DOI: 10.1373/clinchem.2005.052936. View