Serodiagnosis of Fasciolosis by Fast Protein Liquid Chromatography-fractionated Excretory/secretory Antigens

Overview

Journal Parasitol Res

Specialty Parasitology

Date 2016 May 1

PMID 27130320

Citations 6

Authors

Kobra Mokhtarian

Lame Akhlaghi

Ahmad Reza Meamar

Elham Razmjou

Kourosh Manouchehri Naeini

Samaneh Gholami

Masoomeh Najafi Samei

Reza Falak

Affiliations

Soon will be listed here.

Abstract

In several studies, different antigenic preparations and diverse immunological tests were applied for serodiagnosis of Fasciola hepatica infections. Most of these preparations showed cross-reactivity with proteins of other parasites. Application of purified antigens might reduce these cross-reactivities. Here, we used fast protein liquid chromatography (FPLC)-fractionated extracts of F. hepatica excretory/secretory antigens (E/S Ags) for serodiagnosis of human and sheep fasciolosis. To develop an improved diagnostic method, we fractionated F. hepatica E/S Ags by anion exchange chromatography on a Sepharose CL-6B column and then tested the serodiagnostic values of the fractions. We used sera from F. hepatica-infected human and sheep as positive controls. Sera from patients with hydatidosis and strongyloidiasis were used for cross-reactivity studies. Enzyme-linked immunosorbent assays (ELISA) of the second FPLC peak, containing 20, 25, and 70 kDa proteins, discriminated between F. hepatica-infected and uninfected human and sheep samples. Fractionation of F. hepatica E/S Ags by FPLC is a fast and reproducible way of obtaining antigens useful for serodiagnosis of human and sheep fasciolosis with acceptable sensitivity and specificity. Graphical abstract ᅟ.

Citing Articles

Advancement in Diagnosis, Treatment, and Vaccines against : A Comprehensive Review.

Rufino-Moya P, Zafra Leva R, Martinez-Moreno A, Buffoni L, Valderas Garcia E, Perez Arevalo J Pathogens. 2024; 13(8).

PMID: 39204269 PMC: 11357060. DOI: 10.3390/pathogens13080669.

Diagnosis of Human Trematode Infections.

Esteban J, Munoz-Antoli C, Toledo R, Ash L Adv Exp Med Biol. 2024; 1454:541-582.

PMID: 39008275 DOI: 10.1007/978-3-031-60121-7_14.

Recent Developments in Recombinant Proteins for Diagnosis of Human Fascioliasis.

Mirzadeh A, Jafarihaghighi F, Kazemirad E, Sabzevar S, Tanipour M, Ardjmand M Acta Parasitol. 2020; 66(1):13-25.

PMID: 32974849 DOI: 10.1007/s11686-020-00280-5.

Cloning, expression, and spectral analysis of mouse betatrophin.

Gholami S, Goodarzvand Chegini K, Gheibi N, Mokhtarian K, Mohamadi M, Falak R Med J Islam Repub Iran. 2018; 31:102.

PMID: 29951403 PMC: 6014755. DOI: 10.14196/mjiri.31.102.

Comparative assessment of recombinant and native immunogenic forms of Fasciola hepatica proteins for serodiagnosis of sheep fasciolosis.

Mokhtarian K, Meamar A, Khoshmirsafa M, Razmjou E, Masoori L, Khanmohammadi M Parasitol Res. 2017; 117(1):225-232.

PMID: 29199372 DOI: 10.1007/s00436-017-5696-3.

References

Hacariz O, Sayers G, Mulcahy G . A preliminary study to understand the effect of Fasciola hepatica tegument on naïve macrophages and humoral responses in an ovine model. Vet Immunol Immunopathol. 2010; 139(2-4):245-9. DOI: 10.1016/j.vetimm.2010.08.007. View

Yamano K, Goto A, Nakamura-Uchiyama F, Nawa Y, Hada N, Takeda T . Galbeta1-6Gal, antigenic epitope which accounts for serological cross-reaction in diagnosis of Echinococcus multilocularis infection. Parasite Immunol. 2009; 31(8):481-7. DOI: 10.1111/j.1365-3024.2009.01129.x. View

Ashrafi K, Valero M, Panova M, Periago M, Massoud J, Mas-Coma S . Phenotypic analysis of adults of Fasciola hepatica, Fasciola gigantica and intermediate forms from the endemic region of Gilan, Iran. Parasitol Int. 2006; 55(4):249-60. DOI: 10.1016/j.parint.2006.06.003. View

Cornelissen J, Gaasenbeek C, Boersma W, Borgsteede F, van Milligen F . Use of a pre-selected epitope of cathepsin-L1 in a highly specific peptide-based immunoassay for the diagnosis of Fasciola hepatica infections in cattle. Int J Parasitol. 1999; 29(5):685-96. DOI: 10.1016/s0020-7519(99)00017-x. View

Demerdash Z, Diab T, Aly I, Mohamed S, Mahmoud F, Zoheiry M . Diagnostic efficacy of monoclonal antibody based sandwich enzyme linked immunosorbent assay (ELISA) for detection of Fasciola gigantica excretory/secretory antigens in both serum and stool. Parasit Vectors. 2011; 4:176. PMC: 3184274. DOI: 10.1186/1756-3305-4-176. View

Allam A, El-Agamy E, Helmy M . Molecular and immunological characterisation of Fasciola species. Br J Biomed Sci. 2003; 59(4):191-5. DOI: 10.1080/09674845.2002.11783658. View

Abath F, Werkhauser R . The tegument of Schistosoma mansoni: functional and immunological features. Parasite Immunol. 1996; 18(1):15-20. DOI: 10.1046/j.1365-3024.1996.d01-6.x. View

Rokni M, Massoud J, ONeill S, Parkinson M, Dalton J . Diagnosis of human fasciolosis in the Gilan province of Northern Iran: application of cathepsin L-ELISA. Diagn Microbiol Infect Dis. 2002; 44(2):175-9. DOI: 10.1016/s0732-8893(02)00431-5. View

McGonigle S, Dalton J . Isolation of Fasciola hepatica haemoglobin. Parasitology. 1995; 111 ( Pt 2):209-15. DOI: 10.1017/s0031182000064969. View

10.

Rivera Marrero C, Santiago N, Hillyer G . Evaluation of immunodiagnostic antigens in the excretory-secretory products of Fasciola hepatica. J Parasitol. 1988; 74(4):646-52. View

11.

Mohamed M, Al-Sherbiny M, Sharaf A, Elmamlouk T . Immunological identification of Fasciola hepatica antigens containing major human T-cell and B-cell epitopes. J Egypt Soc Parasitol. 2004; 34(3):751-66. View

12.

Robinson M, Menon R, Donnelly S, Dalton J, Ranganathan S . An integrated transcriptomics and proteomics analysis of the secretome of the helminth pathogen Fasciola hepatica: proteins associated with invasion and infection of the mammalian host. Mol Cell Proteomics. 2009; 8(8):1891-907. PMC: 2722771. DOI: 10.1074/mcp.M900045-MCP200. View

13.

Carnevale S, Rodriguez M, Santillan G, Labbe J, Cabrera M, Bellegarde E . Immunodiagnosis of human fascioliasis by an enzyme-linked immunosorbent assay (ELISA) and a micro-ELISA. Clin Diagn Lab Immunol. 2001; 8(1):174-7. PMC: 96029. DOI: 10.1128/CDLI.8.1.174-177.2001. View

14.

Cornelissen J, Gaasenbeek C, Borgsteede F, Holland W, Harmsen M, Boersma W . Early immunodiagnosis of fasciolosis in ruminants using recombinant Fasciola hepatica cathepsin L-like protease. Int J Parasitol. 2001; 31(7):728-37. DOI: 10.1016/s0020-7519(01)00175-8. View

15.

Mas-Coma S, Bargues M, Valero M . Fascioliasis and other plant-borne trematode zoonoses. Int J Parasitol. 2005; 35(11-12):1255-78. DOI: 10.1016/j.ijpara.2005.07.010. View

16.

Sarkari B, Ghobakhloo N, Moshfea A, Eilami O . Seroprevalence of human fasciolosis in a new-emerging focus of fasciolosis in yasuj district, southwest of iran. Iran J Parasitol. 2012; 7(2):15-20. PMC: 3469183. View

17.

Mezo M, Gonzalez-Warleta M, Ubeira F . Optimized serodiagnosis of sheep fascioliasis by Fast-D protein liquid chromatography fractionation of Fasciola hepatica excretory-secretory antigens. J Parasitol. 2003; 89(4):843-9. DOI: 10.1645/GE-74RI.1. View

18.

Valero M, Periago M, Perez-Crespo I, Rodriguez E, Perteguer M, Garate T . Assessing the validity of an ELISA test for the serological diagnosis of human fascioliasis in different epidemiological situations. Trop Med Int Health. 2012; 17(5):630-6. DOI: 10.1111/j.1365-3156.2012.02964.x. View

19.

Da Costa J, da Costa A, Pires M, Lopes S, Castro A, Monjour L . Antigenic components of excretory-secretory products of adult Fasciola hepatica recognized in human infections. Am J Trop Med Hyg. 1996; 54(2):146-8. DOI: 10.4269/ajtmh.1996.54.146. View

20.

Morales A, Espino A . Evaluation and characterization of Fasciola hepatica tegument protein extract for serodiagnosis of human fascioliasis. Clin Vaccine Immunol. 2012; 19(11):1870-8. PMC: 3491547. DOI: 10.1128/CVI.00487-12. View