Bioengineering a 3D Integumentary Organ System from IPS Cells Using an in Vivo Transplantation Model

Overview

Journal Sci Adv

Specialties Biology
Science

Date 2016 Apr 7

PMID 27051874

Citations 42

Authors

Ryoji Takagi

Junko Ishimaru

Ayaka Sugawara

Koh-Ei Toyoshima

Kentaro Ishida

Miho Ogawa

Kei Sakakibara

Kyosuke Asakawa

Akitoshi Kashiwakura

Masamitsu Oshima

Ryohei Minamide

Akio Sato

Toshihiro Yoshitake

Akira Takeda

Hiroshi Egusa

Takashi Tsuji

Affiliations

Soon will be listed here.

Abstract

The integumentary organ system is a complex system that plays important roles in waterproofing, cushioning, protecting deeper tissues, excreting waste, and thermoregulation. We developed a novel in vivo transplantation model designated as a clustering-dependent embryoid body transplantation method and generated a bioengineered three-dimensional (3D) integumentary organ system, including appendage organs such as hair follicles and sebaceous glands, from induced pluripotent stem cells. This bioengineered 3D integumentary organ system was fully functional following transplantation into nude mice and could be properly connected to surrounding host tissues, such as the epidermis, arrector pili muscles, and nerve fibers, without tumorigenesis. The bioengineered hair follicles in the 3D integumentary organ system also showed proper hair eruption and hair cycles, including the rearrangement of follicular stem cells and their niches. Potential applications of the 3D integumentary organ system include an in vitro assay system, an animal model alternative, and a bioengineered organ replacement therapy.

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