The Use of DNA Barcoding on Recently Diverged Species in the Genus Gentiana (Gentianaceae) in China

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2016 Apr 7

PMID 27050315

Citations 13

Authors

Juan Liu

Hai-Fei Yan

Xue-Jun Ge

Affiliations

Soon will be listed here.

Abstract

DNA barcoding of plants poses particular challenges, especially in differentiating, recently diverged taxa. The genus Gentiana (Gentianaceae) is a species-rich plant group which rapidly radiated in the Himalaya-Hengduan Mountains in China. In this study, we tested the core plant barcode (rbcL + matK) and three promising complementary barcodes (trnH-psbA, ITS and ITS2) in 30 Gentiana species across 6 sections using three methods (the genetic distance-based method, Best Close Match and tree-based method). rbcL had the highest PCR efficiency and sequencing success (100%), while the lowest sequence recoverability was from ITS (68.35%). The presence of indels and inversions in trnH-psbA in Gentiana led to difficulties in sequence alignment. When using a single region for analysis, ITS exhibited the highest discriminatory power (60%-74.42%). Of the combinations, matK + ITS provided the highest discrimination success (71.43%-88.24%) and is recommended as the DNA barcode for the genus Gentiana. DNA barcoding proved effective in assigning most species to sections, though it performed poorly in some closely related species in sect. Cruciata because of hybridization events. Our analysis suggests that the status of G. pseudosquarrosa needs to be studied further. The utility of DNA barcoding was also verified in authenticating 'Qin-Jiao' Gentiana medicinal plants (G. macrophylla, G. crassicaulis, G. straminea, and G. dahurica), which can help ensure safe and correct usage of these well-known Chinese traditional medicinal herbs.

Citing Articles

Integrating DNA Barcoding Within an Orthogonal Approach for Herbal Product Authentication: A Narrative Review.

Nazar N, Saxena A, Sebastian A, Slater A, Sundaresan V, Sgamma T Phytochem Anal. 2024; 36(1):7-29.

PMID: 39532481 PMC: 11743069. DOI: 10.1002/pca.3466.

Comparison of plastid genomes and ITS of two sister species in Gentiana and a discussion on potential threats for the endangered species from hybridization.

Mao J, Liang Y, Wang X, Zhang D BMC Plant Biol. 2023; 23(1):101.

PMID: 36800941 PMC: 9940437. DOI: 10.1186/s12870-023-04088-z.

Advancements and future prospective of DNA barcodes in the herbal drug industry.

Mahima K, Sunil Kumar K, Rakhesh K, Rajeswaran P, Sharma A, Sathishkumar R Front Pharmacol. 2022; 13:947512.

PMID: 36339543 PMC: 9635000. DOI: 10.3389/fphar.2022.947512.

DNA barcoding: an efficient technology to authenticate plant species of traditional Chinese medicine and recent advances.

Zhu S, Liu Q, Qiu S, Dai J, Gao X Chin Med. 2022; 17(1):112.

PMID: 36171596 PMC: 9514984. DOI: 10.1186/s13020-022-00655-y.

Sequencing and comparative analysis of chloroplast genomes of three medicinal plants: , and .

Zhao R, Yin S, Xue J, Liu C, Xing Y, Yin H Physiol Mol Biol Plants. 2022; 28(7):1421-1435.

PMID: 36051231 PMC: 9424396. DOI: 10.1007/s12298-022-01217-0.

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