Programmed Cell Death and Clearance of Cell Corpses in Caenorhabditis Elegans

Overview

Journal Cell Mol Life Sci

Publisher Springer

Specialty Biology

Date 2016 Apr 7

PMID 27048817

Citations 30

Authors

Xiaochen Wang

Chonglin Yang

Affiliations

Soon will be listed here.

Abstract

Programmed cell death is critical to the development of diverse animal species from C. elegans to humans. In C. elegans, the cell death program has three genetically distinguishable phases. During the cell suicide phase, the core cell death machinery is activated through a protein interaction cascade. This activates the caspase CED-3, which promotes numerous pro-apoptotic activities including DNA degradation and exposure of the phosphatidylserine "eat me" signal on the cell corpse surface. Specification of the cell death fate involves transcriptional activation of the cell death initiator EGL-1 or the caspase CED-3 by coordinated actions of specific transcription factors in distinct cell types. In the cell corpse clearance stage, recognition of cell corpses by phagocytes triggers several signaling pathways to induce phagocytosis of apoptotic cell corpses. Cell corpse-enclosing phagosomes ultimately fuse with lysosomes for digestion of phagosomal contents. This article summarizes our current knowledge about programmed cell death and clearance of cell corpses in C. elegans.

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References

Nieto C, Almendinger J, Gysi S, Gomez-Orte E, Kaech A, Hengartner M . ccz-1 mediates the digestion of apoptotic corpses in C. elegans. J Cell Sci. 2010; 123(Pt 12):2001-7. DOI: 10.1242/jcs.062331. View

Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A, Nagata S . A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD. Nature. 1998; 391(6662):43-50. DOI: 10.1038/34112. View

Sulston J, Horvitz H . Post-embryonic cell lineages of the nematode, Caenorhabditis elegans. Dev Biol. 1977; 56(1):110-56. DOI: 10.1016/0012-1606(77)90158-0. View

Bohdanowicz M, Grinstein S . Role of phospholipids in endocytosis, phagocytosis, and macropinocytosis. Physiol Rev. 2013; 93(1):69-106. DOI: 10.1152/physrev.00002.2012. View

Shaham S . Identification of multiple Caenorhabditis elegans caspases and their potential roles in proteolytic cascades. J Biol Chem. 1998; 273(52):35109-17. DOI: 10.1074/jbc.273.52.35109. View

Yan N, Gu L, Kokel D, Chai J, Li W, Han A . Structural, biochemical, and functional analyses of CED-9 recognition by the proapoptotic proteins EGL-1 and CED-4. Mol Cell. 2004; 15(6):999-1006. DOI: 10.1016/j.molcel.2004.08.022. View

Chai Y, Tian D, Yang Y, Feng G, Cheng Z, Li W . Apoptotic regulators promote cytokinetic midbody degradation in C. elegans. J Cell Biol. 2012; 199(7):1047-55. PMC: 3529525. DOI: 10.1083/jcb.201209050. View

Abraham M, Lu Y, Shaham S . A morphologically conserved nonapoptotic program promotes linker cell death in Caenorhabditis elegans. Dev Cell. 2007; 12(1):73-86. DOI: 10.1016/j.devcel.2006.11.012. View

Li Z, Venegas V, Nagaoka Y, Morino E, Raghavan P, Audhya A . Necrotic Cells Actively Attract Phagocytes through the Collaborative Action of Two Distinct PS-Exposure Mechanisms. PLoS Genet. 2015; 11(6):e1005285. PMC: 4464654. DOI: 10.1371/journal.pgen.1005285. View

10.

Wu D, Wallen H, Nunez G . Interaction and regulation of subcellular localization of CED-4 by CED-9. Science. 1997; 275(5303):1126-9. DOI: 10.1126/science.275.5303.1126. View

11.

Denning D, Hatch V, Horvitz H . Programmed elimination of cells by caspase-independent cell extrusion in C. elegans. Nature. 2012; 488(7410):226-30. PMC: 3416925. DOI: 10.1038/nature11240. View

12.

Chen Y, Mapes J, Lee E, Skeen-Gaar R, Xue D . Caspase-mediated activation of Caenorhabditis elegans CED-8 promotes apoptosis and phosphatidylserine externalization. Nat Commun. 2013; 4:2726. PMC: 3939056. DOI: 10.1038/ncomms3726. View

13.

Jiang H, Wu Y . LIN-3/EGF promotes the programmed cell death of specific cells in Caenorhabditis elegans by transcriptional activation of the pro-apoptotic gene egl-1. PLoS Genet. 2014; 10(8):e1004513. PMC: 4140636. DOI: 10.1371/journal.pgen.1004513. View

14.

Zou H, Henzel W, Liu X, Lutschg A, Wang X . Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3. Cell. 1997; 90(3):405-13. DOI: 10.1016/s0092-8674(00)80501-2. View

15.

Denning D, Hatch V, Horvitz H . Both the caspase CSP-1 and a caspase-independent pathway promote programmed cell death in parallel to the canonical pathway for apoptosis in Caenorhabditis elegans. PLoS Genet. 2013; 9(3):e1003341. PMC: 3591282. DOI: 10.1371/journal.pgen.1003341. View

16.

Gumienny T, Brugnera E, Kinchen J, Haney L, Nishiwaki K, Walk S . CED-12/ELMO, a novel member of the CrkII/Dock180/Rac pathway, is required for phagocytosis and cell migration. Cell. 2001; 107(1):27-41. DOI: 10.1016/s0092-8674(01)00520-7. View

17.

Hsieh H, Hsu T, Jiang H, Wu Y . Integrin α PAT-2/CDC-42 signaling is required for muscle-mediated clearance of apoptotic cells in Caenorhabditis elegans. PLoS Genet. 2012; 8(5):e1002663. PMC: 3355063. DOI: 10.1371/journal.pgen.1002663. View

18.

Hsu T, Wu Y . Engulfment of apoptotic cells in C. elegans is mediated by integrin alpha/SRC signaling. Curr Biol. 2010; 20(6):477-86. DOI: 10.1016/j.cub.2010.01.062. View

19.

Schertel C, Conradt B . C. elegans orthologs of components of the RB tumor suppressor complex have distinct pro-apoptotic functions. Development. 2007; 134(20):3691-701. DOI: 10.1242/dev.004606. View

20.

Zhou Z, Caron E, Hartwieg E, Hall A, Horvitz H . The C. elegans PH domain protein CED-12 regulates cytoskeletal reorganization via a Rho/Rac GTPase signaling pathway. Dev Cell. 2001; 1(4):477-89. DOI: 10.1016/s1534-5807(01)00058-2. View