Diversity of Staphylococcus Aureus Strains Colonizing Various Niches of the Human Body

Overview

Journal J Infect

Specialty Infectious Diseases

Date 2016 Apr 6

PMID 27045982

Citations 10

Authors

Carol E Muenks

Patrick G Hogan

Jeffrey W Wang

Kimberly A Eisenstein

Carey-Ann D Burnham

Stephanie A Fritz

Affiliations

Soon will be listed here.

Abstract

Objectives: As individuals may be colonized with multiple strains of Staphylococcus aureus at different body sites, the objectives of this study were to determine whether S. aureus polyclonal colonization exists within one body niche and the optimal sampling sites and culture methodology to capture the diversity of S. aureus strains in community-dwelling individuals.

Methods: Swabs were collected from the nares, axillae, and inguinal folds of 3 children with community-associated S. aureus infections and 11 household contacts, all with known S. aureus colonization. S. aureus isolates were recovered from each body niche using 4 culture methods and evaluated for polyclonality using phenotypic and genotypic strain characterization methodologies.

Results: Within individuals, the mean (range) number of phenotypes and genotypes was 2.4 (1-4) and 3.1 (1-6), respectively. Six (43%) and 10 (71%) participants exhibited phenotypic and genotypic polyclonality within one body niche, respectively. Broth enrichment yielded the highest analytical sensitivity for S. aureus recovery, while direct plating to blood agar yielded the highest genotypic strain diversity.

Conclusions: This study revealed S. aureus polyclonality within a single body niche. Culture methodology and sampling sites influenced the analytical sensitivity of S. aureus colonization detection and the robustness of phenotypic and genotypic strain recovery.

Citing Articles

Genomic approach to determine sources of neonatal Staphylococcus aureus infection from carriage in the Gambia.

Bojang A, Chung M, Camara B, Jagne I, Guerillot R, Ndure E BMC Infect Dis. 2024; 24(1):941.

PMID: 39252007 PMC: 11384681. DOI: 10.1186/s12879-024-09837-5.

Genomic Characterization of Methicillin-Susceptible Staphylococcus aureus Carriage in Patients on Home Parenteral Nutrition and Their Caregivers.

Gompelman M, van Weerdenburg I, Wezendonk G, Coolen J, Akkermans R, Rovers C Clin Infect Dis. 2023; 78(5):1285-1288.

PMID: 38011323 PMC: 11093653. DOI: 10.1093/cid/ciad721.

Quantifying patient- and hospital-level antimicrobial resistance dynamics in from routinely collected data.

Leclerc Q, Clements A, Dunn H, Hatcher J, Lindsay J, Grandjean L medRxiv. 2023; .

PMID: 36824943 PMC: 9949191. DOI: 10.1101/2023.02.15.23285946.

Changes of Staphylococcus aureus infection in children before and after the COVID-19 pandemic, Henan, China.

Liang Y, Li J, Hou L, Zhang X, Hou G, Zhang W J Infect. 2022; 86(3):e70-e71.

PMID: 36584772 PMC: 9794387. DOI: 10.1016/j.jinf.2022.12.024.

Transmission and Long-Term Colonization Patterns of in a Nursing Home.

Kasela M, Grzegorczyk A, Korona-Glowniak I, Ossowski M, Nowakowicz-Debek B, Malm A Int J Environ Res Public Health. 2020; 17(21).

PMID: 33147811 PMC: 7672560. DOI: 10.3390/ijerph17218073.

References

Naimi T, LeDell K, Como-Sabetti K, Borchardt S, Boxrud D, Etienne J . Comparison of community- and health care-associated methicillin-resistant Staphylococcus aureus infection. JAMA. 2003; 290(22):2976-84. DOI: 10.1001/jama.290.22.2976. View

Hunter P, Gaston M . Numerical index of the discriminatory ability of typing systems: an application of Simpson's index of diversity. J Clin Microbiol. 1988; 26(11):2465-6. PMC: 266921. DOI: 10.1128/jcm.26.11.2465-2466.1988. View

van Ogtrop M . Effect of broth enrichment cultures on ability to detect carriage of Staphylococcus aureus. Antimicrob Agents Chemother. 1995; 39(9):2169. PMC: 162907. DOI: 10.1128/AAC.39.9.2169. View

Kluytmans J, van Belkum A, Verbrugh H . Nasal carriage of Staphylococcus aureus: epidemiology, underlying mechanisms, and associated risks. Clin Microbiol Rev. 1997; 10(3):505-20. PMC: 172932. DOI: 10.1128/CMR.10.3.505. View

Lina G, Piemont Y, Bes M, Peter M, Gauduchon V, Vandenesch F . Involvement of Panton-Valentine leukocidin-producing Staphylococcus aureus in primary skin infections and pneumonia. Clin Infect Dis. 1999; 29(5):1128-32. DOI: 10.1086/313461. View

Cespedes C, Said-Salim B, Miller M, Lo S, Kreiswirth B, Gordon R . The clonality of Staphylococcus aureus nasal carriage. J Infect Dis. 2005; 191(3):444-52. DOI: 10.1086/427240. View

Lewis 2nd J, Jorgensen J . Inducible clindamycin resistance in Staphylococci: should clinicians and microbiologists be concerned?. Clin Infect Dis. 2005; 40(2):280-5. DOI: 10.1086/426894. View

Hulten K, Kaplan S, Gonzalez B, Hammerman W, Lamberth L, Versalovic J . Three-year surveillance of community onset health care-associated staphylococcus aureus infections in children. Pediatr Infect Dis J. 2006; 25(4):349-53. DOI: 10.1097/01.inf.0000207404.50143.1e. View

Pannaraj P, Hulten K, Gonzalez B, Mason Jr E, Kaplan S . Infective pyomyositis and myositis in children in the era of community-acquired, methicillin-resistant Staphylococcus aureus infection. Clin Infect Dis. 2006; 43(8):953-60. DOI: 10.1086/507637. View

10.

Faden H, Lesse A, Trask J, Hill J, Hess D, Dryja D . Importance of colonization site in the current epidemic of staphylococcal skin abscesses. Pediatrics. 2010; 125(3):e618-24. DOI: 10.1542/peds.2009-1523. View

11.

Lauderdale T, Wang J, Lee W, Huang J, McDonald L, Huang I . Carriage rates of methicillin-resistant Staphylococcus aureus (MRSA) depend on anatomic location, the number of sites cultured, culture methods, and the distribution of clonotypes. Eur J Clin Microbiol Infect Dis. 2010; 29(12):1553-9. DOI: 10.1007/s10096-010-1042-8. View

12.

Mongkolrattanothai K, Gray B, Mankin P, Stanfill A, Pearl R, Wallace L . Simultaneous carriage of multiple genotypes of Staphylococcus aureus in children. J Med Microbiol. 2010; 60(Pt 3):317-322. DOI: 10.1099/jmm.0.025841-0. View

13.

Fritz S, Camins B, Eisenstein K, Fritz J, Epplin E, Burnham C . Effectiveness of measures to eradicate Staphylococcus aureus carriage in patients with community-associated skin and soft-tissue infections: a randomized trial. Infect Control Hosp Epidemiol. 2011; 32(9):872-80. PMC: 3528015. DOI: 10.1086/661285. View

14.

McAllister S, Albrecht V, Fosheim G, Lowery H, Peters P, Gorwitz R . Evaluation of the impact of direct plating, broth enrichment, and specimen source on recovery and diversity of methicillin-resistant Staphylococcus aureus isolates among HIV-infected outpatients. J Clin Microbiol. 2011; 49(12):4126-30. PMC: 3233000. DOI: 10.1128/JCM.05323-11. View

15.

Miller L, Eells S, Taylor A, David M, Ortiz N, Zychowski D . Staphylococcus aureus colonization among household contacts of patients with skin infections: risk factors, strain discordance, and complex ecology. Clin Infect Dis. 2012; 54(11):1523-35. PMC: 3348950. DOI: 10.1093/cid/cis213. View

16.

Fritz S, Hogan P, Hayek G, Eisenstein K, Rodriguez M, Krauss M . Staphylococcus aureus colonization in children with community-associated Staphylococcus aureus skin infections and their household contacts. Arch Pediatr Adolesc Med. 2012; 166(6):551-7. PMC: 3596005. DOI: 10.1001/archpediatrics.2011.900. View

17.

Mernelius S, Lofgren S, Lindgren P, Matussek A . The role of broth enrichment in Staphylococcus aureus cultivation and transmission from the throat to newborn infants: results from the Swedish hygiene intervention and transmission of S. aureus study. Eur J Clin Microbiol Infect Dis. 2013; 32(12):1593-8. PMC: 3825640. DOI: 10.1007/s10096-013-1917-6. View

18.

Rodriguez M, Hogan P, Burnham C, Fritz S . Molecular epidemiology of Staphylococcus aureus in households of children with community-associated S aureus skin and soft tissue infections. J Pediatr. 2013; 164(1):105-11. PMC: 3921902. DOI: 10.1016/j.jpeds.2013.08.072. View

19.

Fritz S, Hogan P, Singh L, Thompson R, Wallace M, Whitney K . Contamination of environmental surfaces with Staphylococcus aureus in households with children infected with methicillin-resistant S aureus. JAMA Pediatr. 2014; 168(11):1030-8. PMC: 4219733. DOI: 10.1001/jamapediatrics.2014.1218. View

20.

Rodriguez M, Hogan P, Satola S, Crispell E, Wylie T, Gao H . Discriminatory Indices of Typing Methods for Epidemiologic Analysis of Contemporary Staphylococcus aureus Strains. Medicine (Baltimore). 2015; 94(37):e1534. PMC: 4635816. DOI: 10.1097/MD.0000000000001534. View