» Articles » PMID: 26983734

Standardized Methods for Detection of Poliovirus Antibodies

Overview
Specialty Molecular Biology
Date 2016 Mar 18
PMID 26983734
Citations 91
Authors
Affiliations
Soon will be listed here.
Abstract

Testing for neutralizing antibodies against polioviruses has been an established gold standard for assessing individual protection from disease, population immunity, vaccine efficacy studies, and other vaccine clinical trials. Detecting poliovirus specific IgM and IgA in sera and mucosal specimens has been proposed for evaluating the status of population mucosal immunity. More recently, there has been a renewed interest in using dried blood spot cards as a medium for sample collection to enhance surveillance of poliovirus immunity. Here, we describe the modified poliovirus microneutralization assay, poliovirus capture IgM and IgA ELISA assays, and dried blood spot polio serology procedures for the detection of antibodies against poliovirus serotypes 1, 2, and 3.

Citing Articles

Post-vaccination campaign evaluation of systemic and mucosal immunity of trivalent oral poliovirus vaccine in Karachi, Pakistan (2020-2021): a cross-sectional study.

Saleem A, Jeyaseelan V, Kazi Z, Zehra M, Alam M, Macklin G Lancet Reg Health Southeast Asia. 2025; 33:100531.

PMID: 39902295 PMC: 11788853. DOI: 10.1016/j.lansea.2025.100531.


Assessing the mucosal intestinal and systemic humoral immunity of sequential schedules of inactivated poliovirus vaccine and bivalent oral poliovirus vaccine for essential immunization in Bangladesh: An open-label, randomized controlled trial.

Snider C, Zaman K, Estivariz C, Aziz A, Yunus M, Haque W Vaccine. 2024; 42(22):126216.

PMID: 39146859 PMC: 11460026. DOI: 10.1016/j.vaccine.2024.126216.


Immunogenicity evaluation of primary polio vaccination schedule with inactivated poliovirus vaccines and bivalent oral poliovirus vaccine.

Xu J, Liu Y, Qiu W, Li W, Hu X, Li X BMC Infect Dis. 2024; 24(1):535.

PMID: 38807038 PMC: 11131326. DOI: 10.1186/s12879-024-09389-8.


The Immunogenicity of Monovalent Oral Poliovirus Vaccine Type 1 (mOPV1) and Inactivated Poliovirus Vaccine (IPV) in the EPI Schedule of India.

Mohanty L, John T, Pawar S, Venkat Ramanan P, Agarkhedkar S, Haldar P Vaccines (Basel). 2024; 12(4).

PMID: 38675806 PMC: 11054616. DOI: 10.3390/vaccines12040424.


Exploring the path to polio eradication: insights from consecutive seroprevalence surveys among Pakistani children.

Hussain I, Umer M, Khan A, Sajid M, Ahmed I, Begum K Front Public Health. 2024; 12:1384410.

PMID: 38601488 PMC: 11004230. DOI: 10.3389/fpubh.2024.1384410.