Pyrosequencing the Midgut Transcriptome of the Banana Weevil Cosmopolites Sordidus (Germar) (Coleoptera: Curculionidae) Reveals Multiple Protease-Like Transcripts

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2016 Mar 8

PMID 26949943

Citations 6

Authors

Arnubio Valencia

Haichuan Wang

Alberto Soto

Manuel Aristizabal

Jorge W Arboleda

Seong-Il Eyun

Daniel D Noriega

Blair Siegfried

Affiliations

Soon will be listed here.

Abstract

The banana weevil Cosmopolites sordidus is an important and serious insect pest in most banana and plantain-growing areas of the world. In spite of the economic importance of this insect pest very little genomic and transcriptomic information exists for this species. In the present study, we characterized the midgut transcriptome of C. sordidus using massive 454-pyrosequencing. We generated over 590,000 sequencing reads that assembled into 30,840 contigs with more than 400 bp, representing a significant expansion of existing sequences available for this insect pest. Among them, 16,427 contigs contained one or more GO terms. In addition, 15,263 contigs were assigned an EC number. In-depth transcriptome analysis identified genes potentially involved in insecticide resistance, peritrophic membrane biosynthesis, immunity-related function and defense against pathogens, and Bacillus thuringiensis toxins binding proteins as well as multiple enzymes involved with protein digestion. This transcriptome will provide a valuable resource for understanding larval physiology and for identifying novel target sites and management approaches for this important insect pest.

Citing Articles

Metagenomic binning of PacBio HiFi data prior to assembly reveals a complete genome of (Germar) (Coleopterea: Curculionidae, Dryophthorinae) the most damaging arthropod pest of bananas and plantains.

Ruiz A, Dam A PeerJ. 2023; 11:e16276.

PMID: 38025758 PMC: 10676084. DOI: 10.7717/peerj.16276.

Post-feeding transcriptomics reveals essential genes expressed in the midgut of the desert locust.

Van Lommel J, Holtof M, Tilleman L, Cools D, Vansteenkiste S, Polgun D Front Physiol. 2023; 14:1232545.

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First Evidence of Feeding-Induced RNAi in Banana Weevil via Exogenous Application of dsRNA.

Mwaka H, Christiaens O, Bwesigye P, Kubiriba J, Tushemereirwe W, Gheysen G Insects. 2022; 13(1).

PMID: 35055882 PMC: 8779063. DOI: 10.3390/insects13010040.

Comparative gut transcriptome analysis of Diatraea saccharalis in response to the dietary source.

Noriega D, Arraes F, Antonino J, Macedo L, Fonseca F, Togawa R PLoS One. 2020; 15(8):e0235575.

PMID: 32745084 PMC: 7398519. DOI: 10.1371/journal.pone.0235575.

Transcriptome and gene expression analysis of three developmental stages of the coffee berry borer, Hypothenemus hampei.

Noriega D, Arias P, Barbosa H, Arraes F, Ossa G, Villegas B Sci Rep. 2019; 9(1):12804.

PMID: 31488852 PMC: 6728347. DOI: 10.1038/s41598-019-49178-x.

References

Johnson K, Rabosky D . Phylogenetic distribution of cysteine proteinases in beetles: evidence for an evolutionary shift to an alkaline digestive strategy in Cerambycidae. Comp Biochem Physiol B Biochem Mol Biol. 2000; 126(4):609-19. DOI: 10.1016/s0305-0491(00)00232-7. View

Eyun S, Wang H, Pauchet Y, Ffrench-Constant R, Benson A, Valencia-Jimenez A . Molecular evolution of glycoside hydrolase genes in the Western corn rootworm (Diabrotica virgifera virgifera). PLoS One. 2014; 9(4):e94052. PMC: 3981738. DOI: 10.1371/journal.pone.0094052. View

Rajagopal R, Sivakumar S, Agrawal N, Malhotra P, Bhatnagar R . Silencing of midgut aminopeptidase N of Spodoptera litura by double-stranded RNA establishes its role as Bacillus thuringiensis toxin receptor. J Biol Chem. 2002; 277(49):46849-51. DOI: 10.1074/jbc.C200523200. View

Zhulidov P, Bogdanova E, Shcheglov A, Vagner L, Khaspekov G, Kozhemyako V . Simple cDNA normalization using kamchatka crab duplex-specific nuclease. Nucleic Acids Res. 2004; 32(3):e37. PMC: 373426. DOI: 10.1093/nar/gnh031. View

NEURATH H . Evolution of proteolytic enzymes. Science. 1984; 224(4647):350-7. DOI: 10.1126/science.6369538. View

Masoud S, Johnson L, White F, Reeck G . Expression of a cysteine proteinase inhibitor (oryzacystatin-I) in transgenic tobacco plants. Plant Mol Biol. 1993; 21(4):655-63. DOI: 10.1007/BF00014548. View

Weis W, Taylor M, Drickamer K . The C-type lectin superfamily in the immune system. Immunol Rev. 1998; 163:19-34. DOI: 10.1111/j.1600-065x.1998.tb01185.x. View

Richards S, Liu Y, Bettencourt B, Hradecky P, Letovsky S, Nielsen R . Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution. Genome Res. 2005; 15(1):1-18. PMC: 540289. DOI: 10.1101/gr.3059305. View

Arakane Y, Muthukrishnan S, Beeman R, Kanost M, Kramer K . Laccase 2 is the phenoloxidase gene required for beetle cuticle tanning. Proc Natl Acad Sci U S A. 2005; 102(32):11337-42. PMC: 1183588. DOI: 10.1073/pnas.0504982102. View

10.

Conesa A, Gotz S, Garcia-Gomez J, Terol J, Talon M, Robles M . Blast2GO: a universal tool for annotation, visualization and analysis in functional genomics research. Bioinformatics. 2005; 21(18):3674-6. DOI: 10.1093/bioinformatics/bti610. View

11.

Merzendorfer H . Insect chitin synthases: a review. J Comp Physiol B. 2005; 176(1):1-15. DOI: 10.1007/s00360-005-0005-3. View

12.

. Insights into social insects from the genome of the honeybee Apis mellifera. Nature. 2006; 443(7114):931-49. PMC: 2048586. DOI: 10.1038/nature05260. View

13.

Hunt T, Bergsten J, Levkanicova Z, Papadopoulou A, St John O, Wild R . A comprehensive phylogeny of beetles reveals the evolutionary origins of a superradiation. Science. 2007; 318(5858):1913-6. DOI: 10.1126/science.1146954. View

14.

Denell R, Gibbs R, Muzny D, Weinstock G, Attaway T, Bell S . The genome of the model beetle and pest Tribolium castaneum. Nature. 2008; 452(7190):949-55. DOI: 10.1038/nature06784. View

15.

Zou Z, Najar F, Wang Y, Roe B, Jiang H . Pyrosequence analysis of expressed sequence tags for Manduca sexta hemolymph proteins involved in immune responses. Insect Biochem Mol Biol. 2008; 38(6):677-82. PMC: 2517850. DOI: 10.1016/j.ibmb.2008.03.009. View

16.

Shendure J, Ji H . Next-generation DNA sequencing. Nat Biotechnol. 2008; 26(10):1135-45. DOI: 10.1038/nbt1486. View

17.

Reddy G, Cruz Z, Guerrero A . Development of an efficient pheromone-based trapping method for the banana root borer Cosmopolites sordidus. J Chem Ecol. 2009; 35(1):111-7. DOI: 10.1007/s10886-008-9580-6. View

18.

Pauchet Y, Wilkinson P, van Munster M, Augustin S, Pauron D, Ffrench-Constant R . Pyrosequencing of the midgut transcriptome of the poplar leaf beetle Chrysomela tremulae reveals new gene families in Coleoptera. Insect Biochem Mol Biol. 2009; 39(5-6):403-13. DOI: 10.1016/j.ibmb.2009.04.001. View

19.

Gibbons J, Janson E, Hittinger C, Johnston M, Abbot P, Rokas A . Benchmarking next-generation transcriptome sequencing for functional and evolutionary genomics. Mol Biol Evol. 2009; 26(12):2731-44. DOI: 10.1093/molbev/msp188. View

20.

Zagrobelny M, Scheibye-Alsing K, Jensen N, Moller B, Gorodkin J, Bak S . 454 pyrosequencing based transcriptome analysis of Zygaena filipendulae with focus on genes involved in biosynthesis of cyanogenic glucosides. BMC Genomics. 2009; 10:574. PMC: 2791780. DOI: 10.1186/1471-2164-10-574. View