Structural Characterization of NRAS Isoform 5

Overview

Journal Protein Sci

Specialty Biochemistry

Date 2016 Mar 8

PMID 26947772

Citations 5

Authors

Joseph Markowitz

Tapas K Mal

Chunhua Yuan

Nicholas B Courtney

Mitra Patel

Andrew R Stiff

James Blachly

Christopher Walker

Ann-Kathrin Eisfeld

Albert de la Chapelle

William E Carson 3rd

Affiliations

Soon will be listed here.

Abstract

It was recently discovered that the NRAS isoform 5 (20 amino acids) is expressed in melanoma and results in a more aggressive cell phenotype. This novel isoform is responsible for increased phosphorylation of downstream targets such as AKT, MEK, and ERK as well as increased cellular proliferation. This structure report describes the NMR solution structure of NRAS isoform 5 to be used as a starting point to understand its biophysical interactions. The isoform is highly flexible in aqueous solution, but forms a helix-turn-coil structure in the presence of trifluoroethanol as determined by NMR and CD spectroscopy.

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