Multiplexed Protein-DNA Cross-linking: Scrunching in Transcription Start Site Selection

Overview

Journal Science

Specialty Science

Date 2016 Mar 5

PMID 26941320

Citations 41

Authors

Jared T Winkelman

Irina O Vvedenskaya

Yuanchao Zhang

Yu Zhang

Jeremy G Bird

Deanne M Taylor

Richard L Gourse

Richard H Ebright

Bryce E Nickels

Affiliations

Soon will be listed here.

Abstract

In bacterial transcription initiation, RNA polymerase (RNAP) selects a transcription start site (TSS) at variable distances downstream of core promoter elements. Using next-generation sequencing and unnatural amino acid-mediated protein-DNA cross-linking, we have determined, for a library of 4(10) promoter sequences, the TSS, the RNAP leading-edge position, and the RNAP trailing-edge position. We find that a promoter element upstream of the TSS, the "discriminator," participates in TSS selection, and that, as the TSS changes, the RNAP leading-edge position changes, but the RNAP trailing-edge position does not change. Changes in the RNAP leading-edge position, but not the RNAP trailing-edge position, are a defining hallmark of the "DNA scrunching" that occurs concurrent with RNA synthesis in initial transcription. We propose that TSS selection involves DNA scrunching prior to RNA synthesis.

Citing Articles

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