The Molecular Basis of Polysaccharide Cleavage by Lytic Polysaccharide Monooxygenases

Overview

Journal Nat Chem Biol

Specialties Biochemistry
Biology
Chemistry

Date 2016 Mar 2

PMID 26928935

Citations 128

Authors

Kristian E H Frandsen

Thomas J Simmons

Paul Dupree

Jens-Christian N Poulsen

Glyn R Hemsworth

Luisa Ciano

Esther M Johnston

Morten Tovborg

Katja S Johansen

Pernille von Freiesleben

Laurence Marmuse

Sebastien Fort

Sylvain Cottaz

Hugues Driguez

Bernard Henrissat

Nicolas Lenfant

Floriana Tuna

Amgalanbaatar Baldansuren

Gideon J Davies

Leila Lo Leggio

Paul H Walton

Affiliations

Soon will be listed here.

Abstract

Lytic polysaccharide monooxygenases (LPMOs) are copper-containing enzymes that oxidatively break down recalcitrant polysaccharides such as cellulose and chitin. Since their discovery, LPMOs have become integral factors in the industrial utilization of biomass, especially in the sustainable generation of cellulosic bioethanol. We report here a structural determination of an LPMO-oligosaccharide complex, yielding detailed insights into the mechanism of action of these enzymes. Using a combination of structure and electron paramagnetic resonance spectroscopy, we reveal the means by which LPMOs interact with saccharide substrates. We further uncover electronic and structural features of the enzyme active site, showing how LPMOs orchestrate the reaction of oxygen with polysaccharide chains.

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