TEM-4, a New Plasmid-mediated Beta-lactamase That Hydrolyzes Broad-spectrum Cephalosporins in a Clinical Isolate of Escherichia Coli

Overview

Journal Antimicrob Agents Chemother

Publisher American Society for Microbiology

Specialty Pharmacology

Date 1989 Nov 1

PMID 2692515

Citations 29

Authors

G C Paul

G Gerbaud

A Bure

A M Philippon

B Pangon

P Courvalin

Affiliations

Soon will be listed here.

Abstract

A clinical isolate of Escherichia coli, strain CB-134, recovered in 1986 from an abdominal abscess, exhibited resistance to penams, oxyimino-beta-lactams including broad-spectrum cephalosporins (cefotaxime, ceftriaxone, ceftazidime), and aztreonam but remained susceptible to cephamycins (cefoxitin, cefotetan) and to moxalactam and imipenem. Clavulanate (2 micrograms/ml) restored the susceptibility of the strain to broad-spectrum cephalosporins and aztreonam. A beta-lactamase with an isoelectric point (pI) of 5.9 was detected in strain CB-134, and the corresponding gene was transferred by conjugation to E. coli together with the associated aminoglycoside resistance determinant [AAC(3)-II] and tetracycline, trimethoprim, and sulfonamide resistance. The beta-lactamase efficiently hydrolyzed cefotaxime and ceftriaxone but only moderately hydrolyzed ceftazidime and was inhibited by clavulanate and sulbactam (1 microM) and by anti-TEM-1 and anti-TEM-2 sera. This extended-spectrum beta-lactamase, conferring resistance to cefotaxime, ceftriaxone, ceftazidime, and aztreonam, was comparable to CTX-1 (TEM-3) but differed from it by pI. Agarose gel electrophoresis of the plasmid DNA indicated that this new enzyme was coded by pUD16, a plasmid of 220 kilobases which belongs to the Inc6 incompatibility group. Hybridization with an intragenic probe for TEM-1 revealed that this beta-lactamase derives from TEM-type beta-lactamases and hence it was named TEM-4.

Citing Articles

Rapid Diagnostics of Orthopaedic-Implant-Associated Infections Using Nanopore Shotgun Metagenomic Sequencing on Tissue Biopsies.

Noone J, Helmersen K, Leegaard T, Skramm I, Aamot H Microorganisms. 2021; 9(1).

PMID: 33406597 PMC: 7823515. DOI: 10.3390/microorganisms9010097.

ESBL-producing  and Its Rapid Rise among Healthy People.

Kawamura K, Nagano N, Suzuki M, Wachino J, Kimura K, Arakawa Y Food Saf (Tokyo). 2020; 5(4):122-150.

PMID: 32231938 PMC: 6989193. DOI: 10.14252/foodsafetyfscj.2017011.

Activity of ceftazidime/avibactam against isogenic strains of Escherichia coli containing KPC and SHV β-lactamases with single amino acid substitutions in the Ω-loop.

Winkler M, Papp-Wallace K, Bonomo R J Antimicrob Chemother. 2015; 70(8):2279-86.

PMID: 25957381 PMC: 4500773. DOI: 10.1093/jac/dkv094.

Antibiotic-resistant Klebsiella pneumoniae and Escherichia coli high-risk clones and an IncFII(k) mosaic plasmid hosting Tn1 (blaTEM-4) in isolates from 1990 to 2004.

Rodriguez I, Novais A, Lira F, Valverde A, Curiao T, Luis Martinez J Antimicrob Agents Chemother. 2015; 59(5):2904-8.

PMID: 25691645 PMC: 4394812. DOI: 10.1128/AAC.00296-15.

Quantifying nonspecific TEM beta-lactamase (blaTEM) genes in a wastewater stream.

Lachmayr K, Kerkhof L, DiRienzo A, Cavanaugh C, Ford T Appl Environ Microbiol. 2008; 75(1):203-11.

PMID: 18997031 PMC: 2612200. DOI: 10.1128/AEM.01254-08.

References

CHABBERT Y, Scavizzi M, Witchitz J, GERBAUD G, Bouanchaud D . Incompatibility groups and the classification of fi - resistance factors. J Bacteriol. 1972; 112(2):666-75. PMC: 251473. DOI: 10.1128/jb.112.2.666-675.1972. View

Barthelemy M, Guionie M, Labia R . Beta-lactamases: determination of their isoelectric points. Antimicrob Agents Chemother. 1978; 13(4):695-8. PMC: 352312. DOI: 10.1128/AAC.13.4.695. View

Maniatis T, Jeffrey A, Kleid D . Nucleotide sequence of the rightward operator of phage lambda. Proc Natl Acad Sci U S A. 1975; 72(3):1184-8. PMC: 432491. DOI: 10.1073/pnas.72.3.1184. View

Haas M, Dowding J . Aminoglycoside-modifying enzymes. Methods Enzymol. 1975; 43:611-28. DOI: 10.1016/0076-6879(75)43124-x. View

Mathew A, Harris A, Marshall M, Ross G . The use of analytical isoelectric focusing for detection and identification of beta-lactamases. J Gen Microbiol. 1975; 88(1):169-78. DOI: 10.1099/00221287-88-1-169. View

Southern E . Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975; 98(3):503-17. DOI: 10.1016/s0022-2836(75)80083-0. View

Labia R, Guionie M, Barthelemy M . Properties of three carbenicillin-hydrolysing beta-lactamases (CARB) from Pseudomonas aeruginosa: identification of a new enzyme. J Antimicrob Chemother. 1981; 7(1):49-56. DOI: 10.1093/jac/7.1.49. View

Gerbaud G, Courvalin P . Translocation of sequences encoding antibiotic resistance from the chromosome to a receptor plasmid in Salmonella ordonez. Mol Gen Genet. 1981; 182(3):390-408. DOI: 10.1007/BF00293927. View

Shah P, Stille W . Escherichia coli and Klebsiella pneumoniae strains more susceptible to cefoxitin than to third generation cephalosporins. J Antimicrob Chemother. 1983; 11(6):597-8. DOI: 10.1093/jac/11.6.597. View

10.

Sanders C, Sanders Jr W . Emergence of resistance during therapy with the newer beta-lactam antibiotics: role of inducible beta-lactamases and implications for the future. Rev Infect Dis. 1983; 5(4):639-48. DOI: 10.1093/clinids/5.4.639. View

11.

Philippon A, Paul G, Jacoby G . Properties of PSE-2 beta-lactamase and genetic basis for its production in Pseudomonas aeruginosa. Antimicrob Agents Chemother. 1983; 24(3):362-9. PMC: 185326. DOI: 10.1128/AAC.24.3.362. View

12.

Knothe H, Shah P, Krcmery V, Antal M, Mitsuhashi S . Transferable resistance to cefotaxime, cefoxitin, cefamandole and cefuroxime in clinical isolates of Klebsiella pneumoniae and Serratia marcescens. Infection. 1983; 11(6):315-7. DOI: 10.1007/BF01641355. View

13.

Kliebe C, NIES B, Meyer J, Wiedemann B . Evolution of plasmid-coded resistance to broad-spectrum cephalosporins. Antimicrob Agents Chemother. 1985; 28(2):302-7. PMC: 180236. DOI: 10.1128/AAC.28.2.302. View

14.

Bakken J, Sanders C, Thomson K . Selective ceftazidime resistance in Escherichia coli: association with changes in outer membrane protein. J Infect Dis. 1987; 155(6):1220-5. DOI: 10.1093/infdis/155.6.1220. View

15.

Brun-Buisson C, Legrand P, Philippon A, Montravers F, Ansquer M, Duval J . Transferable enzymatic resistance to third-generation cephalosporins during nosocomial outbreak of multiresistant Klebsiella pneumoniae. Lancet. 1987; 2(8554):302-6. DOI: 10.1016/s0140-6736(87)90891-9. View

16.

Bauernfeind A, Horl G . Novel R-factor borne beta-lactamase of Escherichia coli confering resistance to cephalosporins. Infection. 1987; 15(4):257-9. DOI: 10.1007/BF01644127. View

17.

Sirot D, Sirot J, Labia R, Morand A, Courvalin P, Darfeuille-Michaud A . Transferable resistance to third-generation cephalosporins in clinical isolates of Klebsiella pneumoniae: identification of CTX-1, a novel beta-lactamase. J Antimicrob Chemother. 1987; 20(3):323-34. DOI: 10.1093/jac/20.3.323. View

18.

Sanders C . Chromosomal cephalosporinases responsible for multiple resistance to newer beta-lactam antibiotics. Annu Rev Microbiol. 1987; 41:573-93. DOI: 10.1146/annurev.mi.41.100187.003041. View

19.

Spencer R, Wheat P, Winstanley T, Cox D, Plested S . Novel beta-lactamase in a clinical isolate of Klebsiella pneumoniae conferring unusual resistance to beta-lactam antibiotics. J Antimicrob Chemother. 1987; 20(6):919-21. DOI: 10.1093/jac/20.6.919. View

20.

Kitzis M, Billot-Klein D, Goldstein F, Williamson R, Tran Van Nhieu G, Carlet J . Dissemination of the novel plasmid-mediated beta-lactamase CTX-1, which confers resistance to broad-spectrum cephalosporins, and its inhibition by beta-lactamase inhibitors. Antimicrob Agents Chemother. 1988; 32(1):9-14. PMC: 172088. DOI: 10.1128/AAC.32.1.9. View