Label-free Imaging to Study Phenotypic Behavioural Traits of Cells in Complex Co-cultures

Overview

Journal Sci Rep

Specialty Science

Date 2016 Feb 27

PMID 26915695

Citations 15

Authors

Rakesh Suman

Gabrielle Smith

Kathryn E A Hazel

Richard Kasprowicz

Mark Coles

Peter OToole

Sangeeta Chawla

Affiliations

Soon will be listed here.

Abstract

Time-lapse imaging is a fundamental tool for studying cellular behaviours, however studies of primary cells in complex co-culture environments often requires fluorescent labelling and significant light exposure that can perturb their natural function over time. Here, we describe ptychographic phase imaging that permits prolonged label-free time-lapse imaging of microglia in the presence of neurons and astrocytes, which better resembles in vivo microenvironments. We demonstrate the use of ptychography as an assay to study the phenotypic behaviour of microglial cells in primary neuronal co-cultures through the addition of cyclosporine A, a potent immune-modulator.

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