Optically-Induced Cell Fusion on Cell Pairing Microstructures

Overview

Journal Sci Rep

Specialty Science

Date 2016 Feb 26

PMID 26912054

Citations 5

Authors

Po-Fu Yang

Chih-Hung Wang

Gwo-Bin Lee

Affiliations

Soon will be listed here.

Abstract

Cell fusion is a critical operation for numerous biomedical applications including cell reprogramming, hybridoma formation, cancer immunotherapy, and tissue regeneration. However, unstable cell contact and random cell pairings have limited efficiency and yields when utilizing traditional methods. Furthermore, it is challenging to selectively perform cell fusion within a group of cells. This study reports a new approach called optically-induced cell fusion (OICF), which integrates cell-pairing microstructures with an optically-induced, localized electrical field. By projecting light patterns onto a photoconductive film (hydrogen-rich, amorphous silicon) coated on an indium-tin-oxide (ITO) glass while an alternating current electrical field was applied between two such ITO glass slides, "virtual" electrodes could be generated that could selectively fuse pairing cells. At 10 kHz, a 57% cell paring rate and an 87% fusion efficiency were successfully achieved at a driving voltage of 20 V(pp), suggesting that this new technology could be promising for selective cell fusion within a group of cells.

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