Impaired Angiogenesis in the Enalapril-treated Neonatal Rat Kidney

Overview

Journal Korean J Pediatr

Specialty Pediatrics

Date 2016 Feb 20

PMID 26893598

Citations 3

Authors

Hyung Eun Yim

Kee Hwan Yoo

Eun Soo Bae

Young Sook Hong

Joo Won Lee

Affiliations

Soon will be listed here.

Abstract

Purpose: Nephrogenesis is normally accompanied by a tightly regulated and efficient vascularization. We investigated the effect of angiotensin II inhibition on angiogenesis in the developing rat kidney.

Methods: Newborn rat pups were treated with enalapril (30 mg/kg/day) or vehicle (control) for 7 days after birth. Renal histological changes were checked using Hematoxylin & Eosin staining. We also investigated the intrarenal expression of vascular endothelial growth factor (VEGF)-A, VEGF receptor 1 (VEGFR1), VEGFR2, platelet-derived growth factor (PDGF)-B, and PDGF receptor-β with Western blotting and immunohistochemical staining at postnatal day 8. Expression of the endothelial cell marker CD31 was examined to determine glomerular and peritubular capillary density.

Results: Enalapril-treated rat kidneys showed disrupted tubules and vessels when compared with the control rat kidneys. In the enalapril-treated group, intrarenal VEGF-A protein expression was significantly higher, whereas VEGFR1 protein expression was lower than that in the control group (P<0.05). The expression of VEGFR2, PDGF-B, and PDGF receptor-β was not different between the 2 groups. The increased capillary CD31 expression on the western blots of enalapril-treated rat kidneys indicated that the total endothelial cell protein level was increased, while the cortical capillary density, assessed using CD31 immunohistochemical staining, was decreased.

Conclusion: Impaired VEGF-VEGFR signaling and altered capillary repair may play a role in the deterioration of the kidney vasculature after blocking of angiotensin II during renal development.

Citing Articles

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Effect of Calcitriol on the Renal Microvasculature Differentiation Disturbances Induced by AT Blockade During Nephrogenesis in Rats.

Deluque A, de Almeida L, Francescato H, da Silva C, Costa R, Antunes-Rodrigues J Front Med (Lausanne). 2020; 7:23.

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Capillary rarefaction and altered renal development: the imbalance between pro- and anti-angiogenic factors in response to angiotensin II inhibition in the developing rat kidney.

Yoo K, Yim H, Bae E, Hong Y J Mol Histol. 2018; 49(2):219-228.

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