Simultaneous Quantitative Analysis of Nine Vitamin D Compounds in Human Blood Using LC-MS/MS

Overview

Journal Bioanalysis

Specialty Chemistry

Date 2016 Feb 20

PMID 26893268

Citations 4

Authors

Nur Sofiah Abu Kassim

Fabio P Gomes

Paul Nicholas Shaw

Amitha K Hewavitharana

Affiliations

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Abstract

Aim: It has been suggested that each member of the family of vitamin D compounds may have different function(s). Therefore, selective quantification of each compound is important in clinical research.

Materials & Methods: Development and validation attempts of a simultaneous determination method of 12 vitamin D compounds in human blood using precolumn derivatization followed by LC-MS/MS is described. Internal standard calibration with 12 stable isotope labeled analogs was used to correct for matrix effects in MS detector.

Results & Conclusion: Nine vitamin D compounds were quantifiable in blood samples with detection limits within femtomole levels. Serum (compared with plasma) was found to be a more suitable sample type, and protein precipitation (compared with saponification) a more effective extraction method for vitamin D assay.

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A novel LC-MS/MS method combined with derivatization for simultaneous quantification of vitamin D metabolites in human serum with diabetes as well as hyperlipidemia.

Wang X, Qin Q, Li F, Fu Y, Liu N RSC Adv. 2023; 13(48):34157-34166.

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The Impact of Sample Type on Vitamin D Quantification and Clinical Classification during Pregnancy.

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Higashi T, Yokota M, Goto A, Komatsu K, Sugiura T, Ogawa S Mass Spectrom (Tokyo). 2016; 5(Spec Iss):S0051.

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