Evaluation of MRNA Expression Levels of Cyp51A and Mdr1, Candidate Genes for Voriconazole Resistance in Aspergillus Flavus

Overview

Journal Jundishapur J Microbiol

Publisher Brieflands

Specialty Microbiology

Date 2016 Feb 12

PMID 26865941

Citations 8

Authors

Azam Fattahi

Farideh Zaini

Parivash Kordbacheh

Sasan Rezaie

Mahin Safara

Roohollah Fateh

Shirin Farahyar

Ali Kanani

Mansour Heidari

Affiliations

Soon will be listed here.

Abstract

Background: Voriconazole Resistance (VRC-R) in Aspergillus flavus isolates impacts the management of aspergillosis, since azoles are the first choice for prophylaxis and therapy. However, to the best of our knowledge, the mechanisms underlying voriconazole resistance are poorly understood.

Objectives: The present study was designed to evaluate mRNA expression levels of cyp51A and mdr1 genes in voriconazole resistant A. flavus by a Real-Time Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) technique.

Materials And Methods: Five A. flavus isolates with resistance to VRC were examined by a RT-PCR approach.

Results: Four out of five isolates revealed cyp51A and mdr1 mRNA overexpression. Interestingly, the isolate, which was negative for cyp51A and mdr1 mRNA expression showed a high voriconazole Minimum Inhibitory Concentration (MIC). Furthermore, a computational-based analysis predicted that voriconazole resistance could be mediated through cooperation with a network protein interaction.

Conclusions: Our experimental and in silico findings may provide new insight in the complex molecular pathways of drug resistance and also could assist design an efficient therapeutic strategy for aspergillosis treatment.

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