Food Color Induced Hepatotoxicity in Swiss Albino Rats, Rattus Norvegicus

Overview

Journal Toxicol Int

Date 2016 Feb 11

PMID 26862277

Citations 10

Authors

Beenam Saxena

Shiv Sharma

Affiliations

Soon will be listed here.

Abstract

Objective: Certain dietary constituents can induce toxicity and play a critical role in the development of several hepatic disorders. Tartrazine, metanil yellow and sunset yellow are widely used azo dyes in food products, so the present study is aimed to investigate the food color induced hepatotoxicity in Swiss albino rats.

Materials And Methods: Swiss albino rats were divided into four groups, each group having six animals. Group I served as control, Group II, Group III and Group IV were administered with 25, 50 and 75 mg/kg body weight blend of sunset yellow, metanil yellow and tartrazine for 30 days. Hepatotoxicity in rats treated with a blend of these food colors was studied by assessing parameters such as serum total protein, serum albumin, serum alkaline phosphatase (ALP) as well as hepatic malondialdehyde (MDA). The activity of superoxide dismutase (SOD), reduced glutathione (GSH) and catalase (CAT) were assessed.

Results: Significantly increased concentrations of serum total protein, serum albumin, serum ALP and hepatic MDA and significantly lowered levels of SOD, reduced GSH and CAT in the liver tissue of treated animals were observed when compared with control animals. The alteration in the liver includes necrosis of hepatocytes, infiltration and vacuolation.

Conclusion: The result indicates that consumption of food color in diet induces liver tissue damage. The used doses of food color were mostly attributable to hepatocellular damage and drastic alteration in antioxidant defense system.

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References

Escobar J, Rubio M, Lissi E . Sod and catalase inactivation by singlet oxygen and peroxyl radicals. Free Radic Biol Med. 1996; 20(3):285-90. DOI: 10.1016/0891-5849(95)02037-3. View

Inal M, Kanbak G, Sunal E . Antioxidant enzyme activities and malondialdehyde levels related to aging. Clin Chim Acta. 2001; 305(1-2):75-80. DOI: 10.1016/s0009-8981(00)00422-8. View

Jain S, McVie R, Duett J, Herbst J . Erythrocyte membrane lipid peroxidation and glycosylated hemoglobin in diabetes. Diabetes. 1989; 38(12):1539-43. DOI: 10.2337/diab.38.12.1539. View

Augustyniak A, Waszkiewicz E, Skrzydlewska E . Preventive action of green tea from changes in the liver antioxidant abilities of different aged rats intoxicated with ethanol. Nutrition. 2005; 21(9):925-32. DOI: 10.1016/j.nut.2005.01.006. View

Sidhom G, Awadallah R, Mahdy K . Physiological effects of some synthetic food colouring additives on rats. Boll Chim Farm. 1998; 136(10):615-27. View

Amin K, Abdel Hameid 2nd H, Abd Elsttar A . Effect of food azo dyes tartrazine and carmoisine on biochemical parameters related to renal, hepatic function and oxidative stress biomarkers in young male rats. Food Chem Toxicol. 2010; 48(10):2994-9. DOI: 10.1016/j.fct.2010.07.039. View

Marklund S, Marklund G . Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem. 1974; 47(3):469-74. DOI: 10.1111/j.1432-1033.1974.tb03714.x. View

Lee K, Chan C, Yang Y, Hsieh Y, Huang Y, Lin H . Aliskiren attenuates chronic carbon tetrachloride-induced liver injury in mice. Eur J Clin Invest. 2012; 42(12):1261-71. DOI: 10.1111/j.1365-2362.2012.02725.x. View

Sayed A, Morcos M . Thymoquinone decreases AGE-induced NF-kappaB activation in proximal tubular epithelial cells. Phytother Res. 2007; 21(9):898-9. DOI: 10.1002/ptr.2177. View

10.

Sinha A . Colorimetric assay of catalase. Anal Biochem. 1972; 47(2):389-94. DOI: 10.1016/0003-2697(72)90132-7. View

11.

Fukuzawa Y, Watanabe Y, Inaguma D, Hotta N . Evaluation of glomerular lesion and abnormal urinary findings in OLETF rats resulting from a long-term diabetic state. J Lab Clin Med. 1996; 128(6):568-78. DOI: 10.1016/s0022-2143(96)90129-8. View

12.

Al-Malki A, Sayed A . Bees' honey attenuation of metanil-yellow-induced hepatotoxicity in rats. Evid Based Complement Alternat Med. 2013; 2013:614580. PMC: 3684022. DOI: 10.1155/2013/614580. View

13.

Dixit S, Purshottam S, Khanna S, Das M . Usage pattern of synthetic food colours in different states of India and exposure assessment through commodities preferentially consumed by children. Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2011; 28(8):996-1005. DOI: 10.1080/19440049.2011.580011. View

14.

Moron M, Depierre J, Mannervik B . Levels of glutathione, glutathione reductase and glutathione S-transferase activities in rat lung and liver. Biochim Biophys Acta. 1979; 582(1):67-78. DOI: 10.1016/0304-4165(79)90289-7. View