All-carbon Multi-electrode Array for Real-time in Vitro Measurements of Oxidizable Neurotransmitters

Overview

Journal Sci Rep

Specialty Science

Date 2016 Feb 10

PMID 26857940

Citations 10

Authors

Federico Picollo

Alfio Battiato

Ettore Bernardi

Marilena Plaitano

Claudio Franchino

Sara Gosso

Alberto Pasquarelli

Emilio Carbone

Paolo Olivero

Valentina Carabelli

Affiliations

Soon will be listed here.

Abstract

We report on the ion beam fabrication of all-carbon multi electrode arrays (MEAs) based on 16 graphitic micro-channels embedded in single-crystal diamond (SCD) substrates. The fabricated SCD-MEAs are systematically employed for the in vitro simultaneous amperometric detection of the secretory activity from populations of chromaffin cells, demonstrating a new sensing approach with respect to standard techniques. The biochemical stability and biocompatibility of the SCD-based device combined with the parallel recording of multi-electrodes array allow: i) a significant time saving in data collection during drug screening and/or pharmacological tests over a large number of cells, ii) the possibility of comparing altered cell functionality among cell populations, and iii) the repeatition of acquisition runs over many cycles with a fully non-toxic and chemically robust bio-sensitive substrate.

Citing Articles

Enhancing the Study of Quantal Exocytotic Events: Combining Diamond Multi-Electrode Arrays with Amperometric PEak Analysis (APE) an Automated Analysis Code.

Tomagra G, Re A, Varzi V, Apra P, Britel A, Franchino C Biosensors (Basel). 2023; 13(12).

PMID: 38131793 PMC: 10741388. DOI: 10.3390/bios13121033.

Multielectrode Arrays as a Means to Study Exocytosis in Human Platelets.

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Quantal Release Analysis of Electrochemically Active Molecules Using Single-Cell Amperometry.

Machado J, Montenegro P, Dominguez N Methods Mol Biol. 2022; 2565:203-211.

PMID: 36205896 DOI: 10.1007/978-1-0716-2671-9_14.

On-Chip Cyclic Voltammetry Measurements Using a Compact 1024-Electrode CMOS IC.

Huang M, Dorta-Quinones C, Minch B, Lindau M Anal Chem. 2021; 93(22):8027-8034.

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Advanced real-time recordings of neuronal activity with tailored patch pipettes, diamond multi-electrode arrays and electrochromic voltage-sensitive dyes.

Kuhn B, Picollo F, Carabelli V, Rispoli G Pflugers Arch. 2020; 473(1):15-36.

PMID: 33047171 PMC: 7782438. DOI: 10.1007/s00424-020-02472-4.

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