LncRNA-mRNA Expression Profiles of Osteoclast After Conditional Knockout HIF-1α

Overview

Journal Front Genet

Date 2022 Jul 8

PMID 35801079

Authors

Yuanye Tian

Qi Shao

Jiahong Gu

Yi Tang

Miaomiao Bie

Yangyifan Zhou

Chunan Cheng

Yi Liang

Qian Zhang

Feiwu Kang

Affiliations

Soon will be listed here.

Abstract

Osteoclasts, which are multinucleated cells formed by monocyte fusion, play a key role in bone resorption. Hypoxia-inducible factor (HIF)-1α is vital for the development of osteoclasts in hypoxic environments and during bone resorption. However, additional research is required to further study the HIF-1α-dependent regulation of osteoclast differentiation at the genetic level. In our study, RNA sequencing (RNA-seq) was used to identify the expression profiles of long noncoding RNAs (lncRNAs) and mRNAs in conditional HIF-1α-knockout osteoclasts. A total of 1,320 mRNAs and 95 lncRNAs were differentially expressed. The expression of lncRNAs MSTRG.7566.12 and MSTRG.31769.2 were strongly negatively correlated with that of Mmp9, Ctsk, etc. Our research provides a basis for further understanding the role of mRNAs and lncRNAs in conditional HIF-1α-knockout osteoclasts, and many of these molecules may be potential targets for treating bone diseases related to HIF-1α.

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