A Novel Technique for in Vitro Maturation of Sheep Oocytes in a Liquid Marble Microbioreactor

Overview

Journal J Assist Reprod Genet

Publisher Springer

Specialties Genetics
Reproductive Medicine

Date 2016 Feb 8

PMID 26852233

Citations 19

Authors

S Ledda

A Idda

J Kelly

F Ariu

L Bogliolo

D Bebbere

Affiliations

Soon will be listed here.

Abstract

Purpose: The aim of this work was to develop a microbioreactor using liquid marble (LM) as a novel system for oocyte in vitro maturation (IVM) in small volumes.

Methods: Cumulus-oocyte complexes (COCs) obtained from slaughterhouse sheep ovaries were in vitro matured in a LM system prepared by placing a drop (30 μl containing 10 COCs) suspended in TCM 199 supplemented with 10 % (v/v) oestrus sheep serum (OSS) and 0.1 IU FSH and LH onto a polytetrafluoroethylene (PTFE) particle bed (LM group). As a control group (CTRL group), COCs were in vitro matured in standard volume and conditions (600 μl of IVM medium in a four-well dish). After 24-h culture at 38.5 °C in 5 % CO2 in air, COCs were released from LM and the following parameters were evaluated: (a) percentage of MII oocytes, (b) oocyte developmental competence following in vitro fertilization (IVF) or parthenogenetic activation (PA) and embryo culture for 8 days in synthetic oviductal fluid (SOF) medium at 38.5 °C in 5 % O2, 5 % CO2, and 90 % N2.

Results: The results indicated similar percentage of MII oocytes in LM and CTRL groups (88.0 vs. 92.0 %). No differences were observed in blastocyst rate after IVF (LM 47.5 % vs. CTRL 50.2 %, P=0.637) or PA (LM 44.4 % vs. CTRL 48.3 %, P=0.426).

Conclusions: The results indicate that LM microbioreactor is a viable technique that provides a suitable microenvironment to induce oocyte in vitro maturation.

Citing Articles

Effect of Liquid Marble 3D Culture System on In Vitro Maturation and Embryo Development of Prepubertal Goat Oocytes.

Podda A, Dujickova L, Ariu F, Leoni G, Izquierdo D, Paramio M Animals (Basel). 2025; 15(2).

PMID: 39858188 PMC: 11758309. DOI: 10.3390/ani15020188.

3D culture applied to reproduction in females: possibilities and perspectives.

Ferronato G, Vit F, da Silveira J Anim Reprod. 2024; 21(1):e20230039.

PMID: 38510565 PMC: 10954237. DOI: 10.1590/1984-3143-AR2023-0039.

New Technique for Human Sperm Cryopreservation Using Emptied Sheep's Ovarian Follicles.

Hussein Zwamel A, Muhammad-Rashad Fakhrildin M, Hadi Hassani H Arch Razi Inst. 2023; 78(2):721-727.

PMID: 37396739 PMC: 10314265. DOI: 10.22092/ARI.2022.359719.2460.

Alternative Culture Systems for Bovine Oocyte In Vitro Maturation: Liquid Marbles and Differentially Shaped 96-Well Plates.

Fernandez-Montoro A, Angel-Velez D, Benedetti C, Azari-Dolatabad N, Pascottini O, Van Soom A Animals (Basel). 2023; 13(10).

PMID: 37238065 PMC: 10215113. DOI: 10.3390/ani13101635.

Bovine in vitro oocyte maturation and embryo culture in liquid marbles 3D culture system.

Ferronato G, Dos Santos C, Rosa P, Bridi A, Perecin F, Meirelles F PLoS One. 2023; 18(4):e0284809.

PMID: 37083878 PMC: 10121032. DOI: 10.1371/journal.pone.0284809.

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