Fluorescent Rhodamines and Fluorogenic Carbopyronines for Super-Resolution STED Microscopy in Living Cells

Overview

Journal Angew Chem Int Ed Engl

Specialty Chemistry

Date 2016 Feb 5

PMID 26844929

Citations 71

Authors

Alexey N Butkevich

Gyuzel Yu Mitronova

Sven C Sidenstein

Jessica L Klocke

Dirk Kamin

Dirk N H Meineke

Elisa DEste

Philip-Tobias Kraemer

Johann G Danzl

Vladimir N Belov

Stefan W Hell

Affiliations

Soon will be listed here.

Abstract

A range of bright and photostable rhodamines and carbopyronines with absorption maxima in the range of λ=500-630 nm were prepared, and enabled the specific labeling of cytoskeletal filaments using HaloTag technology followed by staining with 1 μm solutions of the dye-ligand conjugates. The synthesis, photophysical parameters, fluorogenic behavior, and structure-property relationships of the new dyes are discussed. Light microscopy with stimulated emission depletion (STED) provided one- and two-color images of living cells with an optical resolution of 40-60 nm.

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