Human Umbilical Cord Blood-Derived Serum for Culturing the Supportive Feeder Cells of Human Pluripotent Stem Cell Lines

Overview

Journal Stem Cells Int

Publisher Wiley

Specialty Cell Biology

Date 2016 Feb 4

PMID 26839561

Citations 2

Authors

Ruttachuk Rungsiwiwut

Praewphan Ingrungruanglert

Pranee Numchaisrika

Pramuan Virutamasen

Tatsanee Phermthai

Kamthorn Pruksananonda

Affiliations

Soon will be listed here.

Abstract

Although human pluripotent stem cells (hPSCs) can proliferate robustly on the feeder-free culture system, genetic instability of hPSCs has been reported in such environment. Alternatively, feeder cells enable hPSCs to maintain their pluripotency. The feeder cells are usually grown in a culture medium containing fetal bovine serum (FBS) prior to coculture with hPSCs. The use of FBS might limit the clinical application of hPSCs. Recently, human cord blood-derived serum (hUCS) showed a positive effect on culture of mesenchymal stem cells. It is interesting to test whether hUCS can be used for culture of feeder cells of hPSCs. This study was aimed to replace FBS with hUCS for culturing the human foreskin fibroblasts (HFFs) prior to feeder cell preparation. The results showed that HFFs cultured in hUCS-containing medium (HFF-hUCS) displayed fibroblastic features, high proliferation rates, short population doubling times, and normal karyotypes after prolonged culture. Inactivated HFF-hUCS expressed important genes, including Activin A, FGF2, and TGFβ1, which have been implicated in the maintenance of hPSC pluripotency. Moreover, hPSC lines maintained pluripotency, differentiation capacities, and karyotypic stability after being cocultured for extended period with inactivated HFF-hUCS. Therefore, the results demonstrated the benefit of hUCS for hPSCs culture system.

Citing Articles

Regenerative Therapy Using Umbilical Cord Serum.

Maharajan N, Cho G, Choi J, Jang C In Vivo. 2021; 35(2):699-705.

PMID: 33622862 PMC: 8045055. DOI: 10.21873/invivo.12310.

Cord blood serum harvesting by hydroxyethyl starch: a fetal bovine serum alternative in expansion of umbilical cord-derived mesenchymal stem cells.

Salavati Pour M, Vahidi R, Lashkari M, Derakhshani A, Ameri Z, Farsinejad A Cytotechnology. 2020; 72(4):551-567.

PMID: 32601794 PMC: 7450036. DOI: 10.1007/s10616-020-00404-9.

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