Derivation of Human Embryonic Stem Cells in Defined Conditions

Overview

Journal Nat Biotechnol

Specialty Biotechnology

Date 2006 Jan 3

PMID 16388305

Citations 406

Authors

Tenneille E Ludwig

Mark E Levenstein

Jeffrey M Jones

W Travis Berggren

Erika R Mitchen

Jennifer L Frane

Leann J Crandall

Christine A Daigh

Kevin R Conard

Marian S Piekarczyk

Rachel A Llanas

James A Thomson

Affiliations

Soon will be listed here.

Abstract

We have previously reported that high concentrations of basic fibroblast growth factor (bFGF) support feeder-independent growth of human embryonic stem (ES) cells, but those conditions included poorly defined serum and matrix components. Here we report feeder-independent human ES cell culture that includes protein components solely derived from recombinant sources or purified from human material. We describe the derivation of two new human ES cell lines in these defined culture conditions.

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