Characterization of Rapidly Adhering Amniotic Fluid Cells by Combined Immunofluorescence and Phagocytosis Assays

Overview

Journal Am J Hum Genet

Publisher Cell Press

Specialty Genetics

Date 1989 Nov 1

PMID 2683760

Citations 14

Authors

K Polgar

R Adany

G Abel

J Kappelmayer

L Muszbek

Z Papp

Affiliations

Soon will be listed here.

Abstract

Culture of human amniotic-fluid cells from cases of fetal neural tube defects produces a population of rapidly adhering cells that were initially thought to be macrophages and later interpreted to be of neural origin. In this study double and triple labeling systems for the simultaneous detection of glial and macrophage differentiation marker antigens have been used to demonstrate that rapidly adhering cells cannot be considered a homogeneous population but instead represent two distinct cell types. One of these cell populations is of glial origin and shows specific staining for glial fibrillary acidic protein, while the other population is monocyte-derived macrophages which express marker antigens recognized by Leu M3, KiM7, and Dako antimacrophage monoclonal antibodies.

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References

Esiri M, Booss J . Comparison of methods to identify microglial cells and macrophages in the human central nervous system. J Clin Pathol. 1984; 37(2):150-6. PMC: 498670. DOI: 10.1136/jcp.37.2.150. View

Polgar K, Abel G, Sipka S, Laczko J, Papp Z . Amniotic fluid mononuclear phagocytes: phenotypes and functions. Acta Paediatr Hung. 1988; 29(1-2):63-7. View

Polgar K, Sipka S, Abel G, Papp Z . Neutral-red uptake by amniotic-fluid macrophages in neural-tube defects: a rapid test. N Engl J Med. 1984; 310(22):1463-4. DOI: 10.1056/nejm198405313102217. View

Sutherland G, Brock D, Scrimgeour J . Letter: Amniotic-fluid macrophages and anencephaly. Lancet. 1973; 2(7837):1098-9. DOI: 10.1016/s0140-6736(73)92720-7. View

Ogle J, Ogle C, Noel J, Hurtubise P, Alexander J . Studies on the binding of C3b-coated microspheres to human neutrophils. J Immunol Methods. 1985; 76(1):47-62. DOI: 10.1016/0022-1759(85)90480-6. View

Schelper R, ADRIAN Jr E . Monocytes become macrophages; they do not become microglia: a light and electron microscopic autoradiographic study using 125-iododeoxyuridine. J Neuropathol Exp Neurol. 1986; 45(1):1-19. DOI: 10.1097/00005072-198601000-00001. View

Franklin W, Mason D, Pulford K, Falini B, Bliss E, Gatter K . Immunohistological analysis of human mononuclear phagocytes and dendritic cells by using monoclonal antibodies. Lab Invest. 1986; 54(3):322-35. View

McBride W . Amniotic fluid macrophages from normal and malformed fetuses. Prenat Diagn. 1986; 6(3):195-205. DOI: 10.1002/pd.1970060306. View

Gordon S . Biology of the macrophage. J Cell Sci Suppl. 1986; 4:267-86. DOI: 10.1242/jcs.1986.supplement_4.16. View

10.

Polgar K, Abel G, Laczko J, Sipka S, Papp Z . Immunocytochemical characterization of amniotic fluid macrophages in cases of fetal neural tube defects. Am J Clin Pathol. 1987; 87(1):37-42. DOI: 10.1093/ajcp/87.1.37. View

11.

Kreipe H, Radzun H, Parwaresch M, Haislip A, Hansmann M . Ki-M7 monoclonal antibody specific for myelomonocytic cell lineage and macrophages in human. J Histochem Cytochem. 1987; 35(10):1117-26. DOI: 10.1177/35.10.3476670. View

12.

Bignami A, Dahl D . Astrocyte-specific protein and neuroglial differentiation. An immunofluorescence study with antibodies to the glial fibrillary acidic protein. J Comp Neurol. 1974; 153(1):27-38. DOI: 10.1002/cne.901530104. View

13.

Mueller J, Brun del Re G, Buerki H, Keller H, Hess M, COTTIER H . Nonspecific acid esterase activity: a criterion for differentiation of T and B lymphocytes in mouse lymph nodes. Eur J Immunol. 1975; 5(4):270-4. DOI: 10.1002/eji.1830050411. View

14.

Wood G, Gollahon K, Tilzer S, Vats T, Morantz R . The failure of microglia in normal brain to exhibit mononuclear phagocyte markers. J Neuropathol Exp Neurol. 1979; 38(4):369-76. DOI: 10.1097/00005072-197907000-00002. View

15.

Raff M, Fields K, HAKOMORI S, Mirsky R, Pruss R, Winter J . Cell-type-specific markers for distinguishing and studying neurons and the major classes of glial cells in culture. Brain Res. 1979; 174(2):283-308. DOI: 10.1016/0006-8993(79)90851-5. View

16.

Papp Z, Bell J . Uncultured cells in amniotic fluid from normal and abnormal foetuses. Clin Genet. 1979; 16(4):282-90. DOI: 10.1111/j.1399-0004.1979.tb01001.x. View

17.

Sarkar S, Chang H, Porreco R, Jones O . Neural origin of cells in amniotic fluid. Am J Obstet Gynecol. 1980; 136(1):67-72. DOI: 10.1016/0002-9378(80)90566-9. View

18.

AULA P, von Koskull H, Teramo K, Karjalainen O, Virtanen I, Lehto V . Glial origin of rapidly adhering amniotic fluid cells. Br Med J. 1980; 281(6253):1456-7. PMC: 1714839. DOI: 10.1136/bmj.281.6253.1456. View

19.

Cremer M, Schachner M, Cremer T, Schmidt W, Voigtlander T . Demonstration of astrocytes in cultured amniotic fluid cells of three cases with neural-tube defect. Hum Genet. 1981; 56(3):365-70. DOI: 10.1007/BF00274694. View

20.

von Koskull H, Virtanen I, Lehto V, Vartio T, Dahl D, AULA P . Glial and neuronal cells in amniotic fluid of anencephalic pregnancies. Prenat Diagn. 1981; 1(4):259-67. DOI: 10.1002/pd.1970010405. View