Expression and Functional Role of MiR-29b in Renal Cell Carcinoma

Overview

Journal Int J Clin Exp Pathol

Specialty Pathology

Date 2016 Jan 30

PMID 26823729

Citations 9

Authors

Yi Xu

Jianyong Zhu

Zhangming Lei

Lijun Wan

Xiuwen Zhu

Feng Ye

Yanyue Tong

Affiliations

Soon will be listed here.

Abstract

Objectives: microRNAs (miRNAs) play essential roles in many tumors, including renal cell carcinoma (RCC). The aim of the present study was to investigate the expression and functional role of miR-29b in RCC and to identify its target genes.

Methods: We determined the expression of miR-29b in clear cell RCC (ccRCC) tissues and RCC cell lines (786-O, A498, and SN12-PM6) using quantitative real-time PCR (qRT-PCR). The associations between miR-29b expression and clinical pathological parameters and prognosis were explored. Besides, the role of miR-29b in the SN12-PM6 cells proliferation, apoptosis, cycle, and invasion were investigated after transduction with lentivirus vectors. The kines in family member 1B (KIF1B), possible miR-29b target genes, were predicted using bioinformatics approaches, as well as the role in the pathogenesis of RCC.

Results: Elevated expression of miR-29b was found in both tumor tissues and cell lines. High expression of miR-29b was significantly associated with tumor-node-metastasis (TNM) stage (P = 0.026) and the overall survival (P = 0.009) in the ccRCC. Inhibition of miR-29b expression could promote apoptosis, and inhibit proliferation and invasion ability in SN12-PM6 cells. Also, we confirmed that miR-29b could directly regulate the expression of KIF1B at the post transcriptional level.

Conclusion: These data suggest that miR-29b acts as an oncomiR, promoting proliferation and invasion ability through KIF1B suppression, and it might be a potential marker for prognosis of RCC.

Citing Articles

The Role of miR-29s in Human Cancers-An Update.

Nguyen T, Suman K, Nguyen T, Nguyen H, Do D Biomedicines. 2022; 10(9).

PMID: 36140219 PMC: 9495592. DOI: 10.3390/biomedicines10092121.

MicroRNA Signature in Renal Cell Carcinoma.

Ghafouri-Fard S, Shirvani-Farsani Z, Branicki W, Taheri M Front Oncol. 2020; 10:596359.

PMID: 33330087 PMC: 7734191. DOI: 10.3389/fonc.2020.596359.

miR-30b-5p up-regulation related to the dismal prognosis for patients with renal cell cancer.

Zhang C, Pan X, Peng X, Liu K, Wang J, Zhao L J Clin Lab Anal. 2020; 35(2):e23599.

PMID: 33247622 PMC: 7891535. DOI: 10.1002/jcla.23599.

Serum miR-29b as a novel biomarker for glioblastoma diagnosis and prognosis.

Zhong F, Huang T, Leng J Int J Clin Exp Pathol. 2020; 12(11):4106-4112.

PMID: 31933806 PMC: 6949802.

Molecular Mechanisms in Clear Cell Renal Cell Carcinoma: Role of miRNAs and Hypermethylated miRNA Genes in Crucial Oncogenic Pathways and Processes.

Braga E, Fridman M, Loginov V, Dmitriev A, Morozov S Front Genet. 2019; 10:320.

PMID: 31110513 PMC: 6499217. DOI: 10.3389/fgene.2019.00320.

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