Triploid Human Embryonic Stem Cells Derived from Tripronuclear Zygotes Displayed Pluripotency and Trophoblast Differentiation Ability Similar to the Diploid Human Embryonic Stem Cells

Overview

Journal J Reprod Dev

Specialties Biology
Reproductive Medicine

Date 2016 Jan 30

PMID 26821869

Citations 6

Authors

Ruttachuk Rungsiwiwut

Pranee Numchaisrika

Vichuda Ahnonkitpanit

Pramuan Virutamasen

Kamthorn Pruksananonda

Affiliations

Soon will be listed here.

Abstract

Because the diploid human embryonic stem cells (hESCs) can be successfully derived from tripronuclear zygotes thus, they can serve as an alternative source of derivation of normal karyotype hESC lines. The aim of the present study was to compare the pluripotency and trophoblast differentiation ability of hESCs derived from tripronuclear zygotes and diploid hESCs. In the present study, a total of 20 tripronuclear zygotes were cultured; 8 zygotes developed to the blastocyst stage and 1 hESC line was generated. Unlike the previous studies, chromosomal correction of tripronuclear zygotes during derivation of hESCs did not occur. The established line carries 3 sets of chromosomes and showed a numerical aberration. Although the cell line displayed an abnormal chromosome number, it was found the cell line has been shown to be pluripotent with the ability to differentiate into 3 embryonic germ layers both in vitro and in vivo. The expression of X inactive specific transcript (XIST) in mid-passage (passage 42) of undifferentiated triploid hESCs was detected, indicating X chromosome inactivation of the cell line. Moreover, when this cell line was induced to differentiate toward the trophoblast lineage, morphological and functional trophoblast cells were observed, similar to the diploid hESC line.

Citing Articles

Comparison of Four Protocols for In Vitro Differentiation of Human Embryonic Stem Cells into Trophoblast Lineages by BMP4 and Dual Inhibition of Activin/Nodal and FGF2 Signaling.

Anvar Z, Chakchouk I, Sharif M, Mahadevan S, Su L, Anikar S Reprod Sci. 2023; 31(1):173-189.

PMID: 37658178 PMC: 10784360. DOI: 10.1007/s43032-023-01334-5.

Generation, genomic characterization, and differentiation of triploid human embryonic stem cells.

Haim-Abadi G, Golan-Lev T, Koren A, Benvenisty N Stem Cell Reports. 2023; 18(5):1049-1060.

PMID: 37116485 PMC: 10202691. DOI: 10.1016/j.stemcr.2023.04.001.

Stem Cell-Based Trophoblast Models to Unravel the Genetic Causes of Human Miscarriages.

Nikitina T, Lebedev I Cells. 2022; 11(12).

PMID: 35741051 PMC: 9221414. DOI: 10.3390/cells11121923.

Derivation of human triploid trophoblast stem cells.

Kong X, Chen X, Ou S, Wang W, Li R J Assist Reprod Genet. 2022; 39(5):1183-1193.

PMID: 35243570 PMC: 9107551. DOI: 10.1007/s10815-022-02436-w.

Mesenchymal Stem/Stromal-Like Cells from Diploid and Triploid Human Embryonic Stem Cells Display Different Gene Expression Profiles.

Javidpou M, Seifati S, Farashahi-Yazd E, Hajizadeh-Tafti F, Golzadeh J, Akyash F Iran Biomed J. 2021; 25(2):99-105.

PMID: 33465842 PMC: 7921525. DOI: 10.29252/ibj.25.2.99.

References

Ezashi T, Telugu B, Roberts R . Model systems for studying trophoblast differentiation from human pluripotent stem cells. Cell Tissue Res. 2012; 349(3):809-24. PMC: 3429771. DOI: 10.1007/s00441-012-1371-2. View

Barak Y, Kogosowski A, Goldman S, Soffer Y, Gonen Y, Tesarik J . Pregnancy and birth after transfer of embryos that developed from single-nucleated zygotes obtained by injection of round spermatids into oocytes. Fertil Steril. 1998; 70(1):67-70. DOI: 10.1016/s0015-0282(98)00106-x. View

Marchand M, Horcajadas J, Esteban F, McElroy S, Fisher S, Giudice L . Transcriptomic signature of trophoblast differentiation in a human embryonic stem cell model. Biol Reprod. 2011; 84(6):1258-71. DOI: 10.1095/biolreprod.110.086413. View

Huan Q, Gao X, Wang Y, Shen Y, Ma W, Chen Z . Comparative evaluation of human embryonic stem cell lines derived from zygotes with normal and abnormal pronuclei. Dev Dyn. 2009; 239(2):425-38. DOI: 10.1002/dvdy.22175. View

Bradley C, Scott H, Chami O, Peura T, Dumevska B, Schmidt U . Derivation of Huntington's disease-affected human embryonic stem cell lines. Stem Cells Dev. 2010; 20(3):495-502. DOI: 10.1089/scd.2010.0120. View

Pyle A, Lock L, Donovan P . Neurotrophins mediate human embryonic stem cell survival. Nat Biotechnol. 2006; 24(3):344-50. DOI: 10.1038/nbt1189. View

Kim H, Oh S, Park Y, Ahn H, Sung K, Kang M . Methods for derivation of human embryonic stem cells. Stem Cells. 2005; 23(9):1228-33. DOI: 10.1634/stemcells.2004-0296. View

Mitalipova M, Rao R, Hoyer D, Johnson J, Meisner L, Jones K . Preserving the genetic integrity of human embryonic stem cells. Nat Biotechnol. 2005; 23(1):19-20. DOI: 10.1038/nbt0105-19. View

Macas E, Imthurn B, Rosselli M, Keller P . The chromosomal complements of multipronuclear human zygotes resulting from intracytoplasmic sperm injection. Hum Reprod. 1996; 11(11):2496-501. DOI: 10.1093/oxfordjournals.humrep.a019147. View

10.

Biancotti J, Narwani K, Buehler N, Mandefro B, Golan-Lev T, Yanuka O . Human embryonic stem cells as models for aneuploid chromosomal syndromes. Stem Cells. 2010; 28(9):1530-40. DOI: 10.1002/stem.483. View

11.

Pieters M, Dumoulin J, Bras M, Evers J, Geraedts J . Triploidy after in vitro fertilization: cytogenetic analysis of human zygotes and embryos. J Assist Reprod Genet. 1992; 9(1):68-76. DOI: 10.1007/BF01204118. View

12.

Baharvand H, Kazemi Ashtiani S, Taee A, Massumi M, Valojerdi M, Yazdi P . Generation of new human embryonic stem cell lines with diploid and triploid karyotypes. Dev Growth Differ. 2006; 48(2):117-28. DOI: 10.1111/j.1440-169X.2006.00851.x. View

13.

Biancotti J, Narwani K, Mandefro B, Golan-Lev T, Buehler N, Hill D . The in vitro survival of human monosomies and trisomies as embryonic stem cells. Stem Cell Res. 2012; 9(3):218-24. DOI: 10.1016/j.scr.2012.07.002. View

14.

Liu W, Sun X . Skewed X chromosome inactivation in diploid and triploid female human embryonic stem cells. Hum Reprod. 2009; 24(8):1834-43. DOI: 10.1093/humrep/dep126. View

15.

Sun X, Long X, Yin Y, Jiang Y, Chen X, Liu W . Similar biological characteristics of human embryonic stem cell lines with normal and abnormal karyotypes. Hum Reprod. 2008; 23(10):2185-93. PMC: 2538585. DOI: 10.1093/humrep/den137. View

16.

Thomson J, Itskovitz-Eldor J, Shapiro S, Waknitz M, Swiergiel J, Marshall V . Embryonic stem cell lines derived from human blastocysts. Science. 1998; 282(5391):1145-7. DOI: 10.1126/science.282.5391.1145. View

17.

Stefanova V, Grifo J, Hansis C . Derivation of novel genetically diverse human embryonic stem cell lines. Stem Cells Dev. 2011; 21(9):1559-70. DOI: 10.1089/scd.2011.0642. View

18.

Strom S, Rodriguez-Wallberg K, Holm F, Bergstrom R, Eklund L, Stromberg A . No relationship between embryo morphology and successful derivation of human embryonic stem cell lines. PLoS One. 2011; 5(12):e15329. PMC: 3013107. DOI: 10.1371/journal.pone.0015329. View

19.

Reubinoff B, Pera M, Fong C, Trounson A, Bongso A . Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro. Nat Biotechnol. 2000; 18(4):399-404. DOI: 10.1038/74447. View

20.

Lavon N, Narwani K, Golan-Lev T, Buehler N, Hill D, Benvenisty N . Derivation of euploid human embryonic stem cells from aneuploid embryos. Stem Cells. 2008; 26(7):1874-82. DOI: 10.1634/stemcells.2008-0156. View