Similar Biological Characteristics of Human Embryonic Stem Cell Lines with Normal and Abnormal Karyotypes

Overview

Journal Hum Reprod

Publisher Oxford University Press

Specialty Reproductive Medicine

Date 2008 Jul 10

PMID 18611919

Citations 11

Authors

Xiaofang Sun

Xiaolin Long

Yifei Yin

Yonghua Jiang

Xinjie Chen

Weiqiang Liu

Wenhong Zhang

Hongzi Du

Shaoying Li

Yuhong Zheng

Shu Kong

Qianying Pang

Yu Shi

Yulin Huang

Shengchan Huang

Baoping Liao

Guohong Xiao

Weihua Wang

Affiliations

Soon will be listed here.

Abstract

Background: Human embryonic stem cell (hESC) lines derived from poor quality embryos usually have either normal or abnormal karyotypes. However, it is still unclear whether their biological characteristics are similar.

Methods: Seven new hESC lines were established using discarded embryos. Five cell lines had normal karyotype, one was with an unbalanced Robertsonian translocation and one had a triploid karyotype. Their biological characteristics, short tandem repeat loci, HLA typing, differentiation capability and imprinted gene, DNA methylation and X chromosome inactivation status were compared between different cell lines.

Results: All seven hESC lines had similar biological characteristics regardless of karyotype (five normal and two abnormal), such as expression of stage-specific embryonic antigen (SSEA)-4, tumor-rejection antigen (TRA)-1-81 and TRA-1-60 proteins, transcription factor octamer binding protein 4 mRNA, no detectable expression of SSEA-1 protein and high levels of alkaline phosphatase activity. All cell lines were able to undergo differentiation. Imprinted gene expression and DNA methylation were also similar among these cell lines. Non-random X chromosome inactivation patterns were found in XX cell lines.

Conclusions: The present results suggest that hESC lines with abnormal karyotype are also useful experimental materials for cell therapy, developmental biology and genetic research.

Citing Articles

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Mesenchymal Stem/Stromal-Like Cells from Diploid and Triploid Human Embryonic Stem Cells Display Different Gene Expression Profiles.

Javidpou M, Seifati S, Farashahi-Yazd E, Hajizadeh-Tafti F, Golzadeh J, Akyash F Iran Biomed J. 2021; 25(2):99-105.

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Autophagy Promoted the Degradation of Mutant ATXN3 in Neurally Differentiated Spinocerebellar Ataxia-3 Human Induced Pluripotent Stem Cells.

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Triploid human embryonic stem cells derived from tripronuclear zygotes displayed pluripotency and trophoblast differentiation ability similar to the diploid human embryonic stem cells.

Rungsiwiwut R, Numchaisrika P, Ahnonkitpanit V, Virutamasen P, Pruksananonda K J Reprod Dev. 2016; 62(2):167-76.

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Defining differentially methylated regions specific for the acquisition of pluripotency and maintenance in human pluripotent stem cells via microarray.

He W, Kang X, Du H, Song B, Lu Z, Huang Y PLoS One. 2014; 9(9):e108350.

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