Integrated Magneto-Electrochemical Sensor for Exosome Analysis

Overview

Journal ACS Nano

Publisher American Chemical Society

Specialty Biotechnology

Date 2016 Jan 26

PMID 26808216

Citations 132

Authors

Sangmoo Jeong

Jongmin Park

Divya Pathania

Cesar M Castro

Ralph Weissleder

Hakho Lee

Affiliations

Soon will be listed here.

Abstract

Extracellular vesicles, including exosomes, are nanoscale membrane particles that carry molecular information on parental cells. They are being pursued as biomarkers of cancers that are difficult to detect or serially follow. Here we present a compact sensor technology for rapid, on-site exosome screening. The sensor is based on an integrated magneto-electrochemical assay: exosomes are immunomagnetically captured from patient samples and profiled through electrochemical reaction. By combining magnetic enrichment and enzymatic amplification, the approach enables (i) highly sensitive, cell-specific exosome detection and (ii) sensor miniaturization and scale-up for high-throughput measurements. As a proof-of-concept, we implemented a portable, eight-channel device and applied it to screen extracellular vesicles in plasma samples from ovarian cancer patients. The sensor allowed for the simultaneous profiling of multiple protein markers within an hour, outperforming conventional methods in assay sensitivity and speed.

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