Shugoshin Forms a Specialized Chromatin Domain at Subtelomeres That Regulates Transcription and Replication Timing

Overview

Journal Nat Commun

Specialty Biology

Date 2016 Jan 26

PMID 26804021

Citations 25

Authors

Sanki Tashiro

Tetsuya Handa

Atsushi Matsuda

Takuto Ban

Toru Takigawa

Kazumi Miyasato

Kojiro Ishii

Kazuto Kugou

Kunihiro Ohta

Yasushi Hiraoka

Hisao Masukata

Junko Kanoh

Affiliations

Soon will be listed here.

Abstract

A chromosome is composed of structurally and functionally distinct domains. However, the molecular mechanisms underlying the formation of chromatin structure and the function of subtelomeres, the telomere-adjacent regions, remain obscure. Here we report the roles of the conserved centromeric protein Shugoshin 2 (Sgo2) in defining chromatin structure and functions of the subtelomeres in the fission yeast Schizosaccharomyces pombe. We show that Sgo2 localizes at the subtelomeres preferentially during G2 phase and is essential for the formation of a highly condensed subtelomeric chromatin body 'knob'. Furthermore, the absence of Sgo2 leads to the derepression of the subtelomeric genes and premature DNA replication at the subtelomeric late origins. Thus, the subtelomeric specialized chromatin domain organized by Sgo2 represses both transcription and replication to ensure proper gene expression and replication timing.

Citing Articles

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