Cyto-Insectotoxin 1a from Lachesana Tarabaevi Spider Venom Inhibits Chlamydia Trachomatis Infection

Overview

Journal Probiotics Antimicrob Proteins

Publisher Springer

Specialties Infectious Diseases
Microbiology
Nutritional Sciences
Pharmacology

Date 2016 Jan 20

PMID 26782047

Authors

Nadezhda F Polina

Marina M Shkarupeta

Anna S Popenko

Alexander A Vassilevski

Sergey A Kozlov

Eugene V Grishin

Vassili N Lazarev

Vadim M Govorun

Affiliations

Soon will be listed here.

Abstract

Venom of the ant spider Lachesana tarabaevi contains a wide variety of antimicrobial peptides. Among them, a special place belongs to cyto-insectotoxins, a class of cytolytic molecules showing equally potent antimicrobial and insecticidal effects. We tested one of them, CIT 1a, for ability to suppress Chlamydia trachomatis infection. HEK293 cells were transfected with plasmid vectors harboring the cit 1a gene. Controlled expression of the transgene led to a significant decrease in C. trachomatis viability inside the infected cells. Using proteomic and transcriptomic approaches, we found alterations in protein expression patterns and identified differentially expressed genes in transfected cells.

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