Antagonistic Activity of Cellular Components of Potential Probiotic Bacteria, Isolated from the Gut of Labeo Rohita, Against Aeromonas Hydrophila

Overview

Journal Probiotics Antimicrob Proteins

Publisher Springer

Specialties Infectious Diseases
Microbiology
Nutritional Sciences
Pharmacology

Date 2016 Jan 20

PMID 26781682

Citations 12

Authors

Sib Sankar Giri

V Sukumaran

Shib Sankar Sen

J Vinumonia

B Nazeema Banu

Prasant Kumar Jena

Affiliations

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Abstract

The objective of this study was to characterise the antagonistic activity of cellular components of potential probiotic bacteria isolated from the gut of healthy rohu (Labeo rohita), a tropical freshwater fish, against the fish pathogen, Aeromonas hydrophila. Three potential probiotic strains (referred to as R1, R2, and R5) were screened using a well diffusion, and their antagonistic activity against A. hydrophila was determined. Biochemical tests and 16S rRNA gene analysis confirmed that R1, R2, and R5 were Lactobacillus plantarum VSG3, Pseudomonas aeruginosa VSG2, and Bacillus subtilis VSG1, respectively. Four different fractions of cellular components (i.e. the whole-cell product, heat-killed whole-cell product [HKWCP], intracellular product [ICP], and extracellular product) of these selected strains were effective in an in vitro sensitivity test against 6 A. hydrophila strains. Among the cellular components, the ICP of R1, HKWCP of R2, and ICP of R5 exhibited the strongest antagonistic activities, as evidenced by their inhibition zones. The antimicrobial compounds from these selected cellular components were partially purified by thin-layer and high-performance liquid chromatography, and their properties were analysed. The ranges of pH stability of the purified compounds were wide (3.0-10.0), and compounds were thermally stable up to 90 °C. Considering these results, isolated probiotic strains may find potential applications in the prevention and treatment of aquatic aeromonosis.

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References

Lee N, Yeo I, Park J, Kang B, Hahm Y . Isolation and characterization of a novel analyte from Bacillus subtilis SC-8 antagonistic to Bacillus cereus. J Biosci Bioeng. 2010; 110(3):298-303. DOI: 10.1016/j.jbiosc.2010.03.002. View

Ljungh A, Wadstrom T . Lactic acid bacteria as probiotics. Curr Issues Intest Microbiol. 2006; 7(2):73-89. View

Balcazar J, Vendrell D, de Blas I, Ruiz-Zarzuela I, Girones O, Muzquiz J . In vitro competitive adhesion and production of antagonistic compounds by lactic acid bacteria against fish pathogens. Vet Microbiol. 2007; 122(3-4):373-80. DOI: 10.1016/j.vetmic.2007.01.023. View

Weisburg W, Barns S, Pelletier D, Lane D . 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol. 1991; 173(2):697-703. PMC: 207061. DOI: 10.1128/jb.173.2.697-703.1991. View

Pangsomboon K, Kaewnopparat S, Pitakpornpreecha T, Srichana T . Antibacterial activity of a bacteriocin from Lactobacillus paracasei HL32 against Porphyromonas gingivalis. Arch Oral Biol. 2006; 51(9):784-93. DOI: 10.1016/j.archoralbio.2006.03.008. View

Hubert E, Lobos O, Brevis P, Padilla C . Note: purification and characterization of the bacteriocin PsVP-10 produced by Pseudomonas sp. J Appl Microbiol. 1998; 84(5):910-3. DOI: 10.1046/j.1365-2672.1998.00400.x. View

Vendrell D, Balcazar J, de Blas I, Ruiz-Zarzuela I, Girones O, Muzquiz J . Protection of rainbow trout (Oncorhynchus mykiss) from lactococcosis by probiotic bacteria. Comp Immunol Microbiol Infect Dis. 2007; 31(4):337-45. DOI: 10.1016/j.cimid.2007.04.002. View

Motta A, Cannavan F, Tsai S, Brandelli A . Characterization of a broad range antibacterial substance from a new Bacillus species isolated from Amazon basin. Arch Microbiol. 2007; 188(4):367-75. DOI: 10.1007/s00203-007-0257-2. View

Hjelm M, Bergh O, Riaza A, Nielsen J, Melchiorsen J, Jensen S . Selection and identification of autochthonous potential probiotic bacteria from turbot larvae (Scophthalmus maximus) rearing units. Syst Appl Microbiol. 2004; 27(3):360-71. DOI: 10.1078/0723-2020-00256. View

10.

Fuller R . Probiotics in man and animals. J Appl Bacteriol. 1989; 66(5):365-78. View

11.

Vaseeharan B, Ramasamy P . Control of pathogenic Vibrio spp. by Bacillus subtilis BT23, a possible probiotic treatment for black tiger shrimp Penaeus monodon. Lett Appl Microbiol. 2003; 36(2):83-7. DOI: 10.1046/j.1472-765x.2003.01255.x. View

12.

Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View

13.

Abd El-Rhman A, Khattab Y, Shalaby A . Micrococcus luteus and Pseudomonas species as probiotics for promoting the growth performance and health of Nile tilapia, Oreochromis niloticus. Fish Shellfish Immunol. 2009; 27(2):175-80. DOI: 10.1016/j.fsi.2009.03.020. View

14.

Direkbusarakom , Yoshimizu , Ezura , Ruangpan , Danayadol . Vibrio spp., the dominant flora in shrimp hatchery against some fish pathogenic viruses. J Mar Biotechnol. 1999; 6(4):266-267. View

15.

Al-Ajlani M, Sheikh M, Ahmad Z, Hasnain S . Production of surfactin from Bacillus subtilis MZ-7 grown on pharmamedia commercial medium. Microb Cell Fact. 2007; 6:17. PMC: 1894814. DOI: 10.1186/1475-2859-6-17. View