The Matrix Proteins Hasp and Hig Exhibit Segregated Distribution Within Synaptic Clefts and Play Distinct Roles in Synaptogenesis

Overview

Journal J Neurosci

Specialty Neurology

Date 2016 Jan 14

PMID 26758847

Citations 14

Authors

Minoru Nakayama

Emiko Suzuki

Shin-Ichi Tsunoda

Chihiro Hama

Affiliations

Soon will be listed here.

Abstract

Significance Statement: The synapse has been extensively studied because it is essential for neurotransmission. By contrast, the space between the synaptic terminals, the synaptic cleft, is still an undeveloped research area despite its ubiquity in synapses. In fruit fly brains, we obtained evidence that the matrix protein Hasp and the previously identified Hig, both of which are secreted extracellularly, localize predominantly to synaptic clefts of cholinergic synapses, and modulate the levels of nAChR subunits on postsynaptic membranes. However, Hasp and Hig play differential roles in matrix formation and exhibit segregated distribution within synaptic clefts. These results reveal the molecular mechanisms of synaptic matrix construction and illuminate a molecular architecture within synaptic clefts previously unrevealed in any animal species.

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