Analysis of a Four Generation Family Reveals the Widespread Sequence-dependent Maintenance of Allelic DNA Methylation in Somatic and Germ Cells

Overview

Journal Sci Rep

Specialty Science

Date 2016 Jan 14

PMID 26758766

Citations 8

Authors

Aifa Tang

Yi Huang

Zesong Li

Shengqing Wan

Lisha Mou

Guangliang Yin

Ning Li

Jun Xie

Yudong Xia

Xianxin Li

Liya Luo

Junwen Zhang

Shen Chen

Song Wu

Jihua Sun

Xiaojuan Sun

Zhimao Jiang

Jing Chen

Yingrui Li

Jian Wang

Jun Wang

Zhiming Cai

Yaoting Gui

Affiliations

Soon will be listed here.

Abstract

Differential methylation of the homologous chromosomes, a well-known mechanism leading to genomic imprinting and X-chromosome inactivation, is widely reported at the non-imprinted regions on autosomes. To evaluate the transgenerational DNA methylation patterns in human, we analyzed the DNA methylomes of somatic and germ cells in a four-generation family. We found that allelic asymmetry of DNA methylation was pervasive at the non-imprinted loci and was likely regulated by cis-acting genetic variants. We also observed that the allelic methylation patterns for the vast majority of the cis-regulated loci were shared between the somatic and germ cells from the same individual. These results demonstrated the interaction between genetic and epigenetic variations and suggested the possibility of widespread sequence-dependent transmission of DNA methylation during spermatogenesis.

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