Migration of Bacteria in Semisolid Agar

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1989 Sep 1

PMID 2674941

Citations 180

Authors

A J Wolfe

H C Berg

Affiliations

Soon will be listed here.

Abstract

We studied the migration through semisolid agar of chemotactic and nonchemotactic cells of Escherichia coli. While swarms of nonchemotactic cells were generally smaller than those of chemotactic cells, they varied markedly in size and in structure. Cells that failed to tumble or that tumbled incessantly formed the smallest swarms. Cells that tumbled at intermediate frequencies formed much larger swarms, even when deleted for many of the genes known to be required for chemotaxis. Surprisingly, the higher the tumble frequency, the larger the swarms. Microscopic examination revealed that tumbles enable cells to back away from obstructions in the agar. Thus, not all cells that swarm effectively need be chemotactic.

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References

Berg H, Turner L . Movement of microorganisms in viscous environments. Nature. 1979; 278(5702):349-51. DOI: 10.1038/278349a0. View

Kuo S, Koshland Jr D . Roles of cheY and cheZ gene products in controlling flagellar rotation in bacterial chemotaxis of Escherichia coli. J Bacteriol. 1987; 169(3):1307-14. PMC: 211935. DOI: 10.1128/jb.169.3.1307-1314.1987. View

PARKINSON J . Protein phosphorylation in bacterial chemotaxis. Cell. 1988; 53(1):1-2. DOI: 10.1016/0092-8674(88)90478-3. View

Khan S, Macnab R, DeFranco A, Koshland Jr D . Inversion of a behavioral response in bacterial chemotaxis: explanation at the molecular level. Proc Natl Acad Sci U S A. 1978; 75(9):4150-4. PMC: 336069. DOI: 10.1073/pnas.75.9.4150. View

Yamaguchi S, Fujita H, Ishihara A, Aizawa S, Macnab R . Subdivision of flagellar genes of Salmonella typhimurium into regions responsible for assembly, rotation, and switching. J Bacteriol. 1986; 166(1):187-93. PMC: 214575. DOI: 10.1128/jb.166.1.187-193.1986. View

Stocker B . Transduction of flagellar characters in Salmonella. J Gen Microbiol. 1953; 9(3):410-33. DOI: 10.1099/00221287-9-3-410. View

Armstrong J, Adler J . Complementation of nonchemotactic mutants of Escherichia coli. Genetics. 1969; 61(1):61-6. PMC: 1212151. DOI: 10.1093/genetics/61.1.61. View

Armstrong J, Adler J . Location of genes for motility and chemotaxis on the Escherichia coli genetic map. J Bacteriol. 1969; 97(1):156-61. PMC: 249567. DOI: 10.1128/jb.97.1.156-161.1969. View

Borkovich K, Kaplan N, Hess J, Simon M . Transmembrane signal transduction in bacterial chemotaxis involves ligand-dependent activation of phosphate group transfer. Proc Natl Acad Sci U S A. 1989; 86(4):1208-12. PMC: 286655. DOI: 10.1073/pnas.86.4.1208. View

10.

Larsen S, Reader R, Kort E, Tso W, Adler J . Change in direction of flagellar rotation is the basis of the chemotactic response in Escherichia coli. Nature. 1974; 249(452):74-7. DOI: 10.1038/249074a0. View

11.

Joys T, Stocker B . Complementation of non-flagellate Salmonella mutants. J Gen Microbiol. 1965; 41(1):47-55. DOI: 10.1099/00221287-41-1-47. View

12.

Oosawa K, Imae Y . Glycerol and ethylene glycol: members of a new class of repellents of Escherichia coli chemotaxis. J Bacteriol. 1983; 154(1):104-12. PMC: 217436. DOI: 10.1128/jb.154.1.104-112.1983. View

13.

PARKINSON J, Houts S . Isolation and behavior of Escherichia coli deletion mutants lacking chemotaxis functions. J Bacteriol. 1982; 151(1):106-13. PMC: 220204. DOI: 10.1128/jb.151.1.106-113.1982. View

14.

Block S, Segall J, Berg H . Impulse responses in bacterial chemotaxis. Cell. 1982; 31(1):215-26. DOI: 10.1016/0092-8674(82)90421-4. View

15.

Armstrong J, Adler J . Genetics of motility in Escherichia coli: complementation of paralysed mutants. Genetics. 1967; 56(3):363-73. PMC: 1211624. DOI: 10.1093/genetics/56.3.363. View

16.

Adler J . Chemoreceptors in bacteria. Science. 1969; 166(3913):1588-97. DOI: 10.1126/science.166.3913.1588. View

17.

PARKINSON J . Complementation analysis and deletion mapping of Escherichia coli mutants defective in chemotaxis. J Bacteriol. 1978; 135(1):45-53. PMC: 224763. DOI: 10.1128/jb.135.1.45-53.1978. View

18.

Hedblom M, Adler J . Chemotactic response of Escherichia coli to chemically synthesized amino acids. J Bacteriol. 1983; 155(3):1463-6. PMC: 217852. DOI: 10.1128/jb.155.3.1463-1466.1983. View

19.

Wolfe A, Conley M, Kramer T, Berg H . Reconstitution of signaling in bacterial chemotaxis. J Bacteriol. 1987; 169(5):1878-85. PMC: 212038. DOI: 10.1128/jb.169.5.1878-1885.1987. View

20.

LEDERBERG J . Linear Inheritance in Transductional Clones. Genetics. 1956; 41(6):845-71. PMC: 1224368. DOI: 10.1093/genetics/41.6.845. View