D38-cholesterol As a Raman Active Probe for Imaging Intracellular Cholesterol Storage

Overview

Journal J Biomed Opt

Specialties Biomedical Engineering
Ophthalmology

Date 2016 Jan 1

PMID 26719944

Citations 36

Authors

Alba Alfonso-Garcia

Simon G Pfisterer

Howard Riezman

Elina Ikonen

Eric O Potma

Affiliations

Soon will be listed here.

Abstract

We generated a highly deuterated cholesterol analog (D38-cholesterol) and demonstrated its use for selective vibrational imaging of cholesterol storage in mammalian cells. D38-cholesterol produces detectable signals in stimulated Raman scattering (SRS) imaging, is rapidly taken up by cells, and is efficiently metabolized by acyl-CoA cholesterol acyltransferase to form cholesteryl esters. Using hyperspectral SRS imaging of D38-cholesterol, we visualized cholesterol storage in lipid droplets. We found that some lipid droplets accumulated preferentially unesterified D38-cholesterol, whereas others stored D38-cholesteryl esters. In steroidogenic cells, D38-cholesteryl esters and triacylglycerols were partitioned into distinct sets of lipid droplets. Thus, hyperspectral SRS imaging of D38-cholesterol demonstrates a heterogeneous incorporation of neutral lipid species, i.e., free cholesterol, cholesteryl esters, and triacylglycerols, between individual lipid droplets in a cell.

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