Arabidopsis COP1 SUPPRESSOR 2 Represses COP1 E3 Ubiquitin Ligase Activity Through Their Coiled-Coil Domains Association

Overview

Journal PLoS Genet

Specialty Genetics

Date 2015 Dec 30

PMID 26714275

Citations 17

Authors

Dongqing Xu

Fang Lin

Yan Jiang

Junjie Ling

Chamari Hettiarachchi

Christian Tellgren-Roth

Magnus Holm

Ning Wei

Xing Wang Deng

Affiliations

Soon will be listed here.

Abstract

CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1) functions as an E3 ubiquitin ligase and mediates a variety of developmental processes in Arabidopsis by targeting a number of key regulators for ubiquitination and degradation. Here, we identify a novel COP1 interacting protein, COP1 SUPPRESSOR 2 (CSU2). Loss of function mutations in CSU2 suppress the constitutive photomorphogenic phenotype of cop1-6 in darkness. CSU2 directly interacts with COP1 via their coiled-coil domains and is recruited by COP1 into nuclear speckles in living plant cells. Furthermore, CSU2 inhibits COP1 E3 ubiquitin ligase activity in vitro, and represses COP1 mediated turnover of HY5 in cell-free extracts. We propose that in csu2 cop1-6 mutants, the lack of CSU2's repression of COP1 allows the low level of COP1 to exhibit higher activity that is sufficient to prevent accumulation of HY5 in the dark, thus restoring the etiolated phenotype. In addition, CSU2 is required for primary root development under normal light growth condition.

Citing Articles

How Histone Acetyltransferases Shape Plant Photomorphogenesis and UV Response.

Boycheva I, Bonchev G, Manova V, Stoilov L, Vassileva V Int J Mol Sci. 2024; 25(14).

PMID: 39063093 PMC: 11276938. DOI: 10.3390/ijms25147851.

The lowdown on breakdown: Open questions in plant proteolysis.

Eckardt N, Avin-Wittenberg T, Bassham D, Chen P, Chen Q, Fang J Plant Cell. 2024; 36(9):2931-2975.

PMID: 38980154 PMC: 11371169. DOI: 10.1093/plcell/koae193.

Light regulates nuclear detainment of intron-retained transcripts through COP1-spliceosome to modulate photomorphogenesis.

Zhou H, Zeng H, Yan T, Chen S, Fu Y, Qin G Nat Commun. 2024; 15(1):5130.

PMID: 38879536 PMC: 11180117. DOI: 10.1038/s41467-024-49571-9.

The apple BTB protein MdBT2 positively regulates MdCOP1 abundance to repress anthocyanin biosynthesis.

Kang H, Zhang T, Li Y, Lin-Wang K, Espley R, Du Y Plant Physiol. 2022; 190(1):305-318.

PMID: 35674376 PMC: 9434159. DOI: 10.1093/plphys/kiac279.

HY5: A Pivotal Regulator of Light-Dependent Development in Higher Plants.

Xiao Y, Chu L, Zhang Y, Bian Y, Xiao J, Xu D Front Plant Sci. 2022; 12:800989.

PMID: 35111179 PMC: 8801436. DOI: 10.3389/fpls.2021.800989.

References

Lian H, He S, Zhang Y, Zhu D, Zhang J, Jia K . Blue-light-dependent interaction of cryptochrome 1 with SPA1 defines a dynamic signaling mechanism. Genes Dev. 2011; 25(10):1023-8. PMC: 3093117. DOI: 10.1101/gad.2025111. View

Lee J, He K, Stolc V, Lee H, Figueroa P, Gao Y . Analysis of transcription factor HY5 genomic binding sites revealed its hierarchical role in light regulation of development. Plant Cell. 2007; 19(3):731-49. PMC: 1867377. DOI: 10.1105/tpc.106.047688. View

Jang I, Yang J, Seo H, Chua N . HFR1 is targeted by COP1 E3 ligase for post-translational proteolysis during phytochrome A signaling. Genes Dev. 2005; 19(5):593-602. PMC: 551579. DOI: 10.1101/gad.1247205. View

Lu G, Zhang Q, Huang Y, Song J, Tomaino R, Ehrenberger T . Phosphorylation of ETS1 by Src family kinases prevents its recognition by the COP1 tumor suppressor. Cancer Cell. 2014; 26(2):222-34. PMC: 4169234. DOI: 10.1016/j.ccr.2014.06.026. View

Cho S, Ben Chaabane S, Shah P, Poulsen C, Yang S . COP1 E3 ligase protects HYL1 to retain microRNA biogenesis. Nat Commun. 2014; 5:5867. DOI: 10.1038/ncomms6867. View

Bae G, Choi G . Decoding of light signals by plant phytochromes and their interacting proteins. Annu Rev Plant Biol. 2008; 59:281-311. DOI: 10.1146/annurev.arplant.59.032607.092859. View

Luo X, Lin W, Zhu S, Zhu J, Sun Y, Fan X . Integration of light- and brassinosteroid-signaling pathways by a GATA transcription factor in Arabidopsis. Dev Cell. 2010; 19(6):872-83. PMC: 3022420. DOI: 10.1016/j.devcel.2010.10.023. View

Tepperman J, Hudson M, Khanna R, Zhu T, Chang S, Wang X . Expression profiling of phyB mutant demonstrates substantial contribution of other phytochromes to red-light-regulated gene expression during seedling de-etiolation. Plant J. 2004; 38(5):725-39. DOI: 10.1111/j.1365-313X.2004.02084.x. View

Torii K, McNellis T, Deng X . Functional dissection of Arabidopsis COP1 reveals specific roles of its three structural modules in light control of seedling development. EMBO J. 1998; 17(19):5577-87. PMC: 1170886. DOI: 10.1093/emboj/17.19.5577. View

10.

Lau O, Deng X . The photomorphogenic repressors COP1 and DET1: 20 years later. Trends Plant Sci. 2012; 17(10):584-93. DOI: 10.1016/j.tplants.2012.05.004. View

11.

Hoecker U, Quail P . The phytochrome A-specific signaling intermediate SPA1 interacts directly with COP1, a constitutive repressor of light signaling in Arabidopsis. J Biol Chem. 2001; 276(41):38173-8. DOI: 10.1074/jbc.M103140200. View

12.

Jeong R, Chandra-Shekara A, Roy Barman S, Navarre D, Klessig D, Kachroo A . Cryptochrome 2 and phototropin 2 regulate resistance protein-mediated viral defense by negatively regulating an E3 ubiquitin ligase. Proc Natl Acad Sci U S A. 2010; 107(30):13538-43. PMC: 2922132. DOI: 10.1073/pnas.1004529107. View

13.

Pacin M, Legris M, Casal J . Rapid decline in nuclear costitutive photomorphogenesis1 abundance anticipates the stabilization of its target elongated hypocotyl5 in the light. Plant Physiol. 2014; 164(3):1134-8. PMC: 3938608. DOI: 10.1104/pp.113.234245. View

14.

Selvin P . The renaissance of fluorescence resonance energy transfer. Nat Struct Biol. 2000; 7(9):730-4. DOI: 10.1038/78948. View

15.

Tepperman J, Zhu T, Chang H, Wang X, Quail P . Multiple transcription-factor genes are early targets of phytochrome A signaling. Proc Natl Acad Sci U S A. 2001; 98(16):9437-42. PMC: 55439. DOI: 10.1073/pnas.161300998. View

16.

Christie J . Phototropin blue-light receptors. Annu Rev Plant Biol. 2006; 58:21-45. DOI: 10.1146/annurev.arplant.58.032806.103951. View

17.

Voinnet O, Rivas S, Mestre P, Baulcombe D . An enhanced transient expression system in plants based on suppression of gene silencing by the p19 protein of tomato bushy stunt virus. Plant J. 2003; 33(5):949-56. DOI: 10.1046/j.1365-313x.2003.01676.x. View

18.

Chen M, Chory J . Phytochrome signaling mechanisms and the control of plant development. Trends Cell Biol. 2011; 21(11):664-71. PMC: 3205231. DOI: 10.1016/j.tcb.2011.07.002. View

19.

Yu Y, Wang J, Zhang Z, Quan R, Zhang H, Deng X . Ethylene promotes hypocotyl growth and HY5 degradation by enhancing the movement of COP1 to the nucleus in the light. PLoS Genet. 2013; 9(12):e1004025. PMC: 3861121. DOI: 10.1371/journal.pgen.1004025. View

20.

Karayekov E, Sellaro R, Legris M, Yanovsky M, Casal J . Heat shock-induced fluctuations in clock and light signaling enhance phytochrome B-mediated Arabidopsis deetiolation. Plant Cell. 2013; 25(8):2892-906. PMC: 3784587. DOI: 10.1105/tpc.113.114306. View