Comparative Microarray Analysis of Proliferating and Differentiating Murine ENS Progenitor Cells

Overview

Journal Stem Cells Int

Publisher Wiley

Specialty Cell Biology

Date 2015 Dec 24

PMID 26697082

Citations 7

Authors

Peter Helmut Neckel

Roland Mohr

Ying Zhang

Bernhard Hirt

Lothar Just

Affiliations

Soon will be listed here.

Abstract

Postnatal neural progenitor cells of the enteric nervous system are a potential source for future cell replacement therapies of developmental dysplasia like Hirschsprung's disease. However, little is known about the molecular mechanisms driving the homeostasis and differentiation of this cell pool. In this work, we conducted Affymetrix GeneChip experiments to identify differences in gene regulation between proliferation and early differentiation of enteric neural progenitors from neonatal mice. We detected a total of 1333 regulated genes that were linked to different groups of cellular mechanisms involved in cell cycle, apoptosis, neural proliferation, and differentiation. As expected, we found an augmented inhibition in the gene expression of cell cycle progression as well as an enhanced mRNA expression of neuronal and glial differentiation markers. We further found a marked inactivation of the canonical Wnt pathway after the induction of cellular differentiation. Taken together, these data demonstrate the various molecular mechanisms taking place during the proliferation and early differentiation of enteric neural progenitor cells.

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