A CatalyCEST MRI Contrast Agent That Can Simultaneously Detect Two Enzyme Activities

Overview

Journal Chembiochem

Specialty Biochemistry

Date 2015 Dec 24

PMID 26693680

Citations 10

Authors

Gabriela Fernandez-Cuervo

Sanhita Sinharay

Mark D Pagel

Affiliations

Soon will be listed here.

Abstract

The simultaneous detection of multiple enzyme activities can improve the specificity of disease diagnoses. We therefore synthesized and characterized a diamagnetic chemical exchange saturation transfer (CEST) MRI contrast agent that can simultaneously detect two enzyme activities. Sulfatase and esterase enzymes cleave the ligands of the CEST agent, releasing salicylic acid that can be detected with CEST MRI. Importantly, both enzymes are required to activate the agent to produce a CEST MRI contrast, and the CEST agent was stable without enzyme treatment. These results established that this diamagnetic CEST MRI contrast agent is a platform technology with a modular design that can be potentially exploited to detect other combinations of enzyme activities, which can expand the armamentarium of contrast agents for molecular imaging.

Citing Articles

Repurposing Clinical Agents for Chemical Exchange Saturation Transfer Magnetic Resonance Imaging: Current Status and Future Perspectives.

Chen Z, Han Z, Liu G Pharmaceuticals (Basel). 2020; 14(1).

PMID: 33374213 PMC: 7824058. DOI: 10.3390/ph14010011.

Probing Reversible Guest Binding with Hyperpolarized Xe-NMR: Characteristics and Applications for Cucurbit[]urils.

Jayapaul J, Schroder L Molecules. 2020; 25(4).

PMID: 32093412 PMC: 7070628. DOI: 10.3390/molecules25040957.

Chemistry of MRI Contrast Agents: Current Challenges and New Frontiers.

Wahsner J, Gale E, Rodriguez-Rodriguez A, Caravan P Chem Rev. 2018; 119(2):957-1057.

PMID: 30350585 PMC: 6516866. DOI: 10.1021/acs.chemrev.8b00363.

Detection of Alkaline Phosphatase Enzyme Activity with a CatalyCEST MRI Biosensor.

Daryaei I, Ghaffari M, Jones K, Pagel M ACS Sens. 2018; 1(7):857-861.

PMID: 30246144 PMC: 6145488. DOI: 10.1021/acssensors.6b00203.

Assessments of tumor metabolism with CEST MRI.

Goldenberg J, Pagel M NMR Biomed. 2018; 32(10):e3943.

PMID: 29938857 PMC: 7377947. DOI: 10.1002/nbm.3943.

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