Evaluation of Delta-Aminolevulinic Dehydratase Activity, Oxidative Stress Biomarkers, and Vitamin D Levels in Patients with Multiple Sclerosis

Overview

Journal Neurotox Res

Publisher Springer

Specialty Neurology

Date 2015 Dec 23

PMID 26690779

Citations 10

Authors

Carla Roberta Nunes Polachini

Roselia Maria Spanevello

Daniela Zanini

Jucimara Baldissarelli

Luciane Belmonte Pereira

Maria Rosa Chitolina Schetinger

Ivana Beatrice Manica da Cruz

Charles Elias Assmann

Margarete Dulce Bagatini

Vera Maria Morsch

Affiliations

Soon will be listed here.

Abstract

Multiple sclerosis (MS) is an autoimmune neurological disorder of unknown etiology. Oxidative stress and alterations in vitamin D levels have been implicated in the pathophysiology of MS. The aim of this study was to investigate δ-aminolevulinate dehydratase (δ-ALA-D) activity as well as the levels of vitamin D, lipid peroxidation levels, carbonyl protein content, DNA damage, superoxide dismutase (SOD) and catalase (CAT) activities, and the vitamin C, vitamin E, and non-protein thiol (NPSH) content in samples from patients with the relapsing-remitting form of MS (RRMS). The study population consisted of 29 RRMS patients and 29 healthy subjects. Twelve milliliters of blood was obtained from each individual and used for biochemical determinations. The results showed that δ-ALA-D and CAT activities were significantly increased, while SOD activity was decreased in the whole blood of RRMS patients compared to the control group (P < 0.05). In addition, we observed a significant increase in lipid peroxidation, carbonyl protein levels in serum and damaged DNA in leucocytes in RRMS patients compared with the control group (P < 0.05). Nonetheless, the levels of vitamin C, vitamin E, NPSH, and vitamin D were significantly decreased in RRMS patients in relation to the healthy individuals (P < 0.05). In conclusion, our results suggested that the increase in δ-ALA-D activity may be related to the inflammatory and immune process in MS in an attempt to maintain the cellular metabolism and reduce oxidative stress. Moreover, the alterations in the oxidant/antioxidant balance and lower vitamin D levels may contribute to the pathophysiology of MS.

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References

Jentzsch A, Bachmann H, Furst P, Biesalski H . Improved analysis of malondialdehyde in human body fluids. Free Radic Biol Med. 1996; 20(2):251-6. DOI: 10.1016/0891-5849(95)02043-8. View

Haider L, Fischer M, Frischer J, Bauer J, Hoftberger R, Botond G . Oxidative damage in multiple sclerosis lesions. Brain. 2011; 134(Pt 7):1914-24. PMC: 3122372. DOI: 10.1093/brain/awr128. View

Reinhardt K, Weiss S, Rosenbauer J, Gartner J, von Kries R . Multiple sclerosis in children and adolescents: incidence and clinical picture - new insights from the nationwide German surveillance (2009-2011). Eur J Neurol. 2014; 21(4):654-9. DOI: 10.1111/ene.12371. View

Ramagopalan S, Maugeri N, Handunnetthi L, Lincoln M, Orton S, Dyment D . Expression of the multiple sclerosis-associated MHC class II Allele HLA-DRB1*1501 is regulated by vitamin D. PLoS Genet. 2009; 5(2):e1000369. PMC: 2627899. DOI: 10.1371/journal.pgen.1000369. View

Yousefi B, Ahmadi Y, Ghorbanihaghjo A, Faghfoori Z, Irannejad V . Serum arsenic and lipid peroxidation levels in patients with multiple sclerosis. Biol Trace Elem Res. 2014; 158(3):276-9. DOI: 10.1007/s12011-014-9956-0. View

Djordjevic V, Zvezdanovic L, Cosic V . [Oxidative stress in human diseases]. Srp Arh Celok Lek. 2008; 136 Suppl 2:158-65. DOI: 10.2298/sarh08s2158d. View

Hansen L, Warwick W . A fluorometric micromethod for serum vitamins A and E. Tech Bull Regist Med Technol. 1969; 39(3):70-3. View

Hartmann A, Agurell E, Beevers C, Brendler-Schwaab S, Burlinson B, CLAY P . Recommendations for conducting the in vivo alkaline Comet assay. 4th International Comet Assay Workshop. Mutagenesis. 2002; 18(1):45-51. DOI: 10.1093/mutage/18.1.45. View

Ozgocmen S, Bulut S, Ilhan N, Gulkesen A, Ardicoglu O, Ozkan Y . Vitamin D deficiency and reduced bone mineral density in multiple sclerosis: effect of ambulatory status and functional capacity. J Bone Miner Metab. 2005; 23(4):309-13. DOI: 10.1007/s00774-005-0604-9. View

10.

Mazza M, Pomponi M, Janiri L, Bria P, Mazza S . Omega-3 fatty acids and antioxidants in neurological and psychiatric diseases: an overview. Prog Neuropsychopharmacol Biol Psychiatry. 2006; 31(1):12-26. DOI: 10.1016/j.pnpbp.2006.07.010. View

11.

Grober U, Spitz J, Reichrath J, Kisters K, Holick M . Vitamin D: Update 2013: From rickets prophylaxis to general preventive healthcare. Dermatoendocrinol. 2014; 5(3):331-47. PMC: 3908963. DOI: 10.4161/derm.26738. View

12.

Wacker M, Holick M . Vitamin D - effects on skeletal and extraskeletal health and the need for supplementation. Nutrients. 2013; 5(1):111-48. PMC: 3571641. DOI: 10.3390/nu5010111. View

13.

Friese M, Schattling B, Fugger L . Mechanisms of neurodegeneration and axonal dysfunction in multiple sclerosis. Nat Rev Neurol. 2014; 10(4):225-38. DOI: 10.1038/nrneurol.2014.37. View

14.

Johnson F, Giulivi C . Superoxide dismutases and their impact upon human health. Mol Aspects Med. 2005; 26(4-5):340-52. DOI: 10.1016/j.mam.2005.07.006. View

15.

Zanini D, Pelinson L, Schmatz R, Pereira L, Martins C, Baldissareli J . δ-Aminolevulinate dehydratase activity in lung cancer patients and its relationship with oxidative stress. Biomed Pharmacother. 2014; 68(5):603-9. DOI: 10.1016/j.biopha.2014.04.005. View

16.

McDonald W, Compston A, Edan G, Goodkin D, Hartung H, Lublin F . Recommended diagnostic criteria for multiple sclerosis: guidelines from the International Panel on the diagnosis of multiple sclerosis. Ann Neurol. 2001; 50(1):121-7. DOI: 10.1002/ana.1032. View

17.

Paniz C, Bairros A, Valentini J, Charao M, Bulcao R, Moro A . The influence of the serum vitamin C levels on oxidative stress biomarkers in elderly women. Clin Biochem. 2007; 40(18):1367-72. DOI: 10.1016/j.clinbiochem.2007.08.013. View

18.

Ljubisavljevic S, Stojanovic I, Cvetkovic T, Vojinovic S, Stojanov D, Stojanovic D . Erythrocytes' antioxidative capacity as a potential marker of oxidative stress intensity in neuroinflammation. J Neurol Sci. 2013; 337(1-2):8-13. DOI: 10.1016/j.jns.2013.11.006. View

19.

Koch M, Ramsaransing G, Arutjunyan A, Stepanov M, Teelken A, Heersema D . Oxidative stress in serum and peripheral blood leukocytes in patients with different disease courses of multiple sclerosis. J Neurol. 2005; 253(4):483-7. DOI: 10.1007/s00415-005-0037-3. View

20.

Sassa S . ALAD porphyria. Semin Liver Dis. 1998; 18(1):95-101. DOI: 10.1055/s-2007-1007145. View