Transcriptional Analysis of the CDC7 Protein Kinase Gene of Saccharomyces Cerevisiae

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1989 Jul 25

PMID 2668893

Citations 2

Authors

J Ham

D Moore

J Rosamond

I R JOHNSTON

Affiliations

Soon will be listed here.

Abstract

The 5' flanking region of the CDC7 gene of Saccharomyces cerevisiae has been sequenced to a point 797 nucleotides upstream of the putative translational initiation codon (designated +1). The sequence reveals a number of symmetry elements between -100 to -380 and two blocks of high local AT content between -29 to -75 and -112 to -144. Transcription initiates heterogeneously at about 10 discrete sites up to 110 nucleotides upstream of the putative translational initiation codon. Some minor transcriptional start sites were also observed between the ATG at +1 and a second in-frame ATG at +55, suggesting that CDC7 may also be translationally heterogeneous. Deletion analysis of the CDC7 upstream region has shown that the gene lacks a functional TATA box, and has identified a 30bp element that is necessary for normal CDC7 promoter function during mitosis. This motif has significant homology with a component of the c-fos promoter which acts to regulate c-fos expression by binding a transcription activating factor. The results suggest that the 30bp motif may play a similar role in regulating CDC7 expression and that there may be similarities between factors that regulate CDC7 expression in yeast and c-fos expression in vertebrate cells.

Citing Articles

The CDC7 protein of Saccharomyces cerevisiae is a phosphoprotein that contains protein kinase activity.

Yoon H, Campbell J Proc Natl Acad Sci U S A. 1991; 88(9):3574-8.

PMID: 2023904 PMC: 51494. DOI: 10.1073/pnas.88.9.3574.

CDC7 protein kinase activity is required for mitosis and meiosis in Saccharomyces cerevisiae.

Buck V, White A, Rosamond J Mol Gen Genet. 1991; 227(3):452-7.

PMID: 1865880 DOI: 10.1007/BF00273937.

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