Dbx1 Precursor Cells Are a Source of Inspiratory XII Premotoneurons

Overview

Journal Elife

Specialty Biology

Date 2015 Dec 22

PMID 26687006

Citations 34

Authors

Ann L Revill

Nikolas C Vann

Victoria T Akins

Andrew Kottick

Paul A Gray

Christopher A Del Negro

Gregory D Funk

Affiliations

Soon will be listed here.

Abstract

All behaviors require coordinated activation of motoneurons from central command and premotor networks. The genetic identities of premotoneurons providing behaviorally relevant excitation to any pool of respiratory motoneurons remain unknown. Recently, we established in vitro that Dbx1-derived pre-Bötzinger complex neurons are critical for rhythm generation and that a subpopulation serves a premotor function (Wang et al., 2014). Here, we further show that a subpopulation of Dbx1-derived intermediate reticular (IRt) neurons are rhythmically active during inspiration and project to the hypoglossal (XII) nucleus that contains motoneurons important for maintaining airway patency. Laser ablation of Dbx1 IRt neurons, 57% of which are glutamatergic, decreased ipsilateral inspiratory motor output without affecting frequency. We conclude that a subset of Dbx1 IRt neurons is a source of premotor excitatory drive, contributing to the inspiratory behavior of XII motoneurons, as well as a key component of the airway control network whose dysfunction contributes to sleep apnea.

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