Nasopharyngeal Swabs and Nasopharyngeal Aspirates Equally Effective for the Diagnosis of Viral Respiratory Disease in Hospitalized Children

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1989 Jun 1

PMID 2666446

Citations 20

Authors

H Frayha

S Castriciano

J Mahony

M Chernesky

Affiliations

Soon will be listed here.

Abstract

Paired nasopharyngeal swab and nasopharyngeal aspirate specimens from 125 patients were compared for viral diagnosis. The viral isolation rates were comparable for the two types of specimens. There was a high level of agreement between the two specimens in overall positivity rate by immunofluorescence and positivity in culture-confirmed patients.

Citing Articles

Pediatric Respiratory Syncytial Virus Diagnostic Testing Performance: A Systematic Review and Meta-analysis.

Onwuchekwa C, Atwell J, Mora Moreo L, Menon S, Machado B, Siapka M J Infect Dis. 2023; 228(11):1516-1527.

PMID: 37285396 PMC: 10681870. DOI: 10.1093/infdis/jiad185.

Comparison of detection rate of 16 sampling methods for respiratory viruses: a Bayesian network meta-analysis of clinical data and systematic review.

Hou N, Wang K, Zhang H, Bai M, Chen H, Song W BMJ Glob Health. 2020; 5(11).

PMID: 33168521 PMC: 7654123. DOI: 10.1136/bmjgh-2020-003053.

Cost-Effective Respiratory Virus Testing.

Pinsky B, Hayden R J Clin Microbiol. 2019; 57(9).

PMID: 31142607 PMC: 6711893. DOI: 10.1128/JCM.00373-19.

Respiratory Pathogens in Infants Diagnosed with Acute Lower Respiratory Tract Infection in a Tertiary Care Hospital of Western India Using Multiplex Real Time PCR.

Sonawane A, Shastri J, Bavdekar S Indian J Pediatr. 2019; 86(5):433-438.

PMID: 30637585 PMC: 7091426. DOI: 10.1007/s12098-018-2840-8.

Diagnostic Performance of Multiplex Nucleic Acid Testing of Bronchoalveolar Lavage and Bronchial Wash Specimens for Respiratory Viral Pathogens.

Munigala S, Burnham C, Anderson N, Liang S, Lawrence S, Warren D J Clin Microbiol. 2018; 56(10).

PMID: 30093393 PMC: 6156318. DOI: 10.1128/JCM.00973-18.

References

Hughes J, Mann D, Hamparian V . Detection of respiratory syncytial virus in clinical specimens by viral culture, direct and indirect immunofluorescence, and enzyme immunoassay. J Clin Microbiol. 1988; 26(3):588-91. PMC: 266341. DOI: 10.1128/jcm.26.3.588-591.1988. View

Gardner P, MCQUILLIN J, McGuckin R . The late detection of respiratory syncytial virus in cells of respiratory tract by immunofluorescence. J Hyg (Lond). 1970; 68(4):575-80. PMC: 2130874. DOI: 10.1017/s0022172400042509. View

CRUZ J, Quinonez E, de Fernandez A, Peralta F . Isolation of viruses from nasopharyngeal secretions: comparison of aspiration and swabbing as means of sample collection. J Infect Dis. 1987; 156(2):415-6. DOI: 10.1093/infdis/156.2.415. View

Ahluwalia G, Embree J, McNicol P, Law B, Hammond G . Comparison of nasopharyngeal aspirate and nasopharyngeal swab specimens for respiratory syncytial virus diagnosis by cell culture, indirect immunofluorescence assay, and enzyme-linked immunosorbent assay. J Clin Microbiol. 1987; 25(5):763-7. PMC: 266085. DOI: 10.1128/jcm.25.5.763-767.1987. View

Gilbert B, Knight V . Biochemistry and clinical applications of ribavirin. Antimicrob Agents Chemother. 1986; 30(2):201-5. PMC: 180518. DOI: 10.1128/AAC.30.2.201. View

Hall C, DOUGLAS Jr R . Clinically useful method for the isolation of respiratory syncytial virus. J Infect Dis. 1975; 131(1):1-5. DOI: 10.1093/infdis/131.1.1. View

Hendry R, Pierik L, McIntosh K . Comparison of washed nasopharyngeal cells and whole nasal secretions for detection of respiratory syncytial virus antigens by enzyme-linked immunosorbent assay. J Clin Microbiol. 1986; 23(2):383-4. PMC: 268650. DOI: 10.1128/jcm.23.2.383-384.1986. View

Bromberg K, Tannis G, Daidone B, Clarke L, Sierra M . Comparison of ortho respiratory syncytial virus enzyme-linked immunosorbent assay and HEp-2 cell culture. J Clin Microbiol. 1985; 22(6):1071-2. PMC: 271886. DOI: 10.1128/jcm.22.6.1071-1072.1985. View

Mahony J, Chernesky M . Effect of swab type and storage temperature on the isolation of Chlamydia trachomatis from clinical specimens. J Clin Microbiol. 1985; 22(5):865-7. PMC: 268547. DOI: 10.1128/jcm.22.5.865-867.1985. View

10.

Treuhaft M, Soukup J, Sullivan B . Practical recommendations for the detection of pediatric respiratory syncytial virus infections. J Clin Microbiol. 1985; 22(2):270-3. PMC: 268373. DOI: 10.1128/jcm.22.2.270-273.1985. View

11.

Kim H, Wyatt R, Fernie B, BRANDT C, Arrobio J, Jeffries B . Respiratory syncytial virus detection by immunofluorescence in nasal secretions with monoclonal antibodies against selected surface and internal proteins. J Clin Microbiol. 1983; 18(6):1399-404. PMC: 272915. DOI: 10.1128/jcm.18.6.1399-1404.1983. View

12.

Bell D, Walsh E, Hruska J, Schnabel K, Hall C . Rapid detection of respiratory syncytial virus with a monoclonal antibody. J Clin Microbiol. 1983; 17(6):1099-101. PMC: 272809. DOI: 10.1128/jcm.17.6.1099-1101.1983. View

13.

Crane L, Gutterman P, Chapel T, Lerner A . Incubation of swab materials with herpes simplex virus. J Infect Dis. 1980; 141(4):531. DOI: 10.1093/infdis/141.4.531. View

14.

McIntosh K, Hendry R, Fahnestock M, Pierik L . Enzyme-linked immunosorbent assay for detection of respiratory syncytial virus infection: application to clinical samples. J Clin Microbiol. 1982; 16(2):329-33. PMC: 272355. DOI: 10.1128/jcm.16.2.329-333.1982. View

15.

Hall C, McBride J, Walsh E, Bell D, Gala C, Hildreth S . Aerosolized ribavirin treatment of infants with respiratory syncytial viral infection. A randomized double-blind study. N Engl J Med. 1983; 308(24):1443-7. DOI: 10.1056/NEJM198306163082403. View

16.

Arens M, Swierkosz E, Schmidt R, Armstrong T, Rivetna K . Enhanced isolation of respiratory syncytial virus in cell culture. J Clin Microbiol. 1986; 23(4):800-2. PMC: 362843. DOI: 10.1128/jcm.23.4.800-802.1986. View

17.

CRADOCK-WATSON J, MCQUILLIN J, Gardner P . Rapid diagnosis of respiratory syncytial virus infection in children by the immunofluorescent technique. J Clin Pathol. 1971; 24(4):308-12. PMC: 476980. DOI: 10.1136/jcp.24.4.308. View

18.

Masters H, WEBER K, Groothuis J, Wren C, Lauer B . Comparison of nasopharyngeal washings and swab specimens for diagnosis of respiratory syncytial virus by EIA, FAT, and cell culture. Diagn Microbiol Infect Dis. 1987; 8(2):101-5. DOI: 10.1016/0732-8893(87)90156-8. View