Near-infrared Photoactivatable Control of Ca(2+) Signaling and Optogenetic Immunomodulation

Overview

Journal Elife

Specialty Biology

Date 2015 Dec 10

PMID 26646180

Citations 99

Authors

Lian He

Yuanwei Zhang

Guolin Ma

Peng Tan

Zhanjun Li

Shengbing Zang

Xiang Wu

Ji Jing

Shaohai Fang

Lijuan Zhou

Youjun Wang

Yun Huang

Patrick G Hogan

Gang Han

Yubin Zhou

Affiliations

Soon will be listed here.

Abstract

The application of current channelrhodopsin-based optogenetic tools is limited by the lack of strict ion selectivity and the inability to extend the spectra sensitivity into the near-infrared (NIR) tissue transmissible range. Here we present an NIR-stimulable optogenetic platform (termed 'Opto-CRAC') that selectively and remotely controls Ca(2+) oscillations and Ca(2+)-responsive gene expression to regulate the function of non-excitable cells, including T lymphocytes, macrophages and dendritic cells. When coupled to upconversion nanoparticles, the optogenetic operation window is shifted from the visible range to NIR wavelengths to enable wireless photoactivation of Ca(2+)-dependent signaling and optogenetic modulation of immunoinflammatory responses. In a mouse model of melanoma by using ovalbumin as surrogate tumor antigen, Opto-CRAC has been shown to act as a genetically-encoded 'photoactivatable adjuvant' to improve antigen-specific immune responses to specifically destruct tumor cells. Our study represents a solid step forward towards the goal of achieving remote and wireless control of Ca(2+)-modulated activities with tailored function.

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