Cloning of Histidine Genes of Azospirillum Brasilense: Organization of the ABFH Gene Cluster and Nucleotide Sequence of the HisB Gene

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1989 Apr 1

PMID 2664449

Citations 16

Authors

R Fani

M Bazzicalupo

G Damiani

A Bianchi

C Schipani

V Sgaramella

M Polsinelli

Affiliations

Soon will be listed here.

Abstract

A cluster of four Azospirillum brasilense histidine biosynthetic genes, hisA, hisB, hisF and hisH, was identified on a 4.5 kb DNA fragment and its organization studied by complementation analysis of Escherichia coli mutations and nucleotide sequence. The nucleotide sequence of a 1.3 kb fragment that complemented the E. coli hisB mutation was determined and an ORF of 624 nucleotides which can code for a protein of 207 amino acids was identified. A significant base sequence homology with the carboxy-terminal moiety of the E. coli hisB gene (0.53) and the Saccharomyces cerevisiae HIS3 gene (0.44), coding for an imidazole glycerolphosphate dehydratase activity was found. The amino acid sequence and composition, the hydropathic profile and the predicted secondary structures of the yeast, E. coli and A. brasilense proteins were compared. The significance of the data presented is discussed.

Citing Articles

Molecular evolution of hisB genes.

Brilli M, Fani R J Mol Evol. 2004; 58(2):225-37.

PMID: 15042344 DOI: 10.1007/s00239-003-2547-x.

Evolution of the structure and chromosomal distribution of histidine biosynthetic genes.

Fani R, Mori E, Tamburini E, Lazcano A Orig Life Evol Biosph. 1998; 28(4-6):555-70.

PMID: 9742729 DOI: 10.1023/a:1006531526299.

Crystal structures of HINT demonstrate that histidine triad proteins are GalT-related nucleotide-binding proteins.

Brenner C, Garrison P, Gilmour J, Peisach D, Ringe D, Petsko G Nat Struct Biol. 1997; 4(3):231-8.

PMID: 9164465 PMC: 2571075. DOI: 10.1038/nsb0397-231.

Histidine biosynthetic pathway and genes: structure, regulation, and evolution.

Alifano P, Fani R, Lio P, Lazcano A, Bazzicalupo M, Carlomagno M Microbiol Rev. 1996; 60(1):44-69.

PMID: 8852895 PMC: 239417. DOI: 10.1128/mr.60.1.44-69.1996.

Molecular evolution of the histidine biosynthetic pathway.

Fani R, Lio P, Lazcano A J Mol Evol. 1995; 41(6):760-74.

PMID: 8587121 DOI: 10.1007/BF00173156.

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