The Majority of Resorptions in Old Mice Are Euploid

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2015 Dec 5

PMID 26636341

Citations 1

Authors

Yong Tao

X Johne Liu

Affiliations

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Abstract

Chromosomal abnormality is a leading cause of aging-related infertility, spontaneous abortion and congenital birth defects in humans. Karyotype analyses of spontaneously aborted human fetuses reveal high proportions (~50%) being chromosomal abnormal with the majority being trisomies of various chromosomes. As a model organism, mice are widely used for studies of reproduction and reproductive aging. Like older women, older mice exhibit high incidences of early embryo death. However, it is not known if aneuploidy is prevalent amongst resorptions in older mice. We have karyotyped 65 retarded/resorbed fetuses in 10-month-old C57BL/6 mice, and found that 55 (84.6%±8.8%, with 95% confidence) were euploid. Similarly, of 40 such fetuses from 17 month-old C57BL/6 mice, we found 38 (95±7%, with 95% confidence 95%) being euploid. Therefore, aneuploidy is not a leading cause of embryo death in older mice.

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References

Angell R . Predivision in human oocytes at meiosis I: a mechanism for trisomy formation in man. Hum Genet. 1991; 86(4):383-7. DOI: 10.1007/BF00201839. View

Hassold T, Chen N, Funkhouser J, JOOSS T, Manuel B, Matsuura J . A cytogenetic study of 1000 spontaneous abortions. Ann Hum Genet. 1980; 44(2):151-78. DOI: 10.1111/j.1469-1809.1980.tb00955.x. View

Eppig J, Schultz R, OBrien M, Chesnel F . Relationship between the developmental programs controlling nuclear and cytoplasmic maturation of mouse oocytes. Dev Biol. 1994; 164(1):1-9. DOI: 10.1006/dbio.1994.1175. View

FINN C . Embryonic death in aged mice. Nature. 1962; 194:499-500. DOI: 10.1038/194499a0. View

Baird D, Collins J, Egozcue J, Evers L, Gianaroli L, Leridon H . Fertility and ageing. Hum Reprod Update. 2005; 11(3):261-76. DOI: 10.1093/humupd/dmi006. View

Lightfoot D, Kouznetsova A, Mahdy E, Wilbertz J, Hoog C . The fate of mosaic aneuploid embryos during mouse development. Dev Biol. 2005; 289(2):384-94. DOI: 10.1016/j.ydbio.2005.11.001. View

Koehler K, Schrump S, Cherry J, Hassold T, Hunt P . Near-human aneuploidy levels in female mice with homeologous chromosomes. Curr Biol. 2006; 16(15):R579-80. DOI: 10.1016/j.cub.2006.07.018. View

Cukurcam S, Betzendahl I, Michel G, Vogt E, Hegele-Hartung C, Lindenthal B . Influence of follicular fluid meiosis-activating sterol on aneuploidy rate and precocious chromatid segregation in aged mouse oocytes. Hum Reprod. 2006; 22(3):815-28. DOI: 10.1093/humrep/del442. View

Pan H, Ma P, Zhu W, Schultz R . Age-associated increase in aneuploidy and changes in gene expression in mouse eggs. Dev Biol. 2008; 316(2):397-407. PMC: 2374949. DOI: 10.1016/j.ydbio.2008.01.048. View

10.

Vanneste E, Voet T, Le Caignec C, Ampe M, Konings P, Melotte C . Chromosome instability is common in human cleavage-stage embryos. Nat Med. 2009; 15(5):577-83. DOI: 10.1038/nm.1924. View

11.

Haadsma M, Groen H, Mooij T, Burger C, Broekmans F, Lambalk C . Miscarriage risk for IVF pregnancies in poor responders to ovarian hyperstimulation. Reprod Biomed Online. 2010; 20(2):191-200. DOI: 10.1016/j.rbmo.2009.11.005. View

12.

Chiang T, Duncan F, Schindler K, Schultz R, Lampson M . Evidence that weakened centromere cohesion is a leading cause of age-related aneuploidy in oocytes. Curr Biol. 2010; 20(17):1522-8. PMC: 2939204. DOI: 10.1016/j.cub.2010.06.069. View

13.

Kuliev A, Zlatopolsky Z, Kirillova I, Spivakova J, Cieslak Janzen J . Meiosis errors in over 20,000 oocytes studied in the practice of preimplantation aneuploidy testing. Reprod Biomed Online. 2010; 22(1):2-8. DOI: 10.1016/j.rbmo.2010.08.014. View

14.

Massie J, Shahine L, Milki A, Westphal L, Lathi R . Ovarian stimulation and the risk of aneuploid conceptions. Fertil Steril. 2010; 95(3):970-2. DOI: 10.1016/j.fertnstert.2010.07.1088. View

15.

Kroon B, Harrison K, Martin N, Wong B, Yazdani A . Miscarriage karyotype and its relationship with maternal body mass index, age, and mode of conception. Fertil Steril. 2010; 95(5):1827-9. DOI: 10.1016/j.fertnstert.2010.11.065. View

16.

Gabriel A, Thornhill A, Ottolini C, Gordon A, Brown A, Taylor J . Array comparative genomic hybridisation on first polar bodies suggests that non-disjunction is not the predominant mechanism leading to aneuploidy in humans. J Med Genet. 2011; 48(7):433-7. DOI: 10.1136/jmg.2010.088070. View

17.

Selesniemi K, Lee H, Muhlhauser A, Tilly J . Prevention of maternal aging-associated oocyte aneuploidy and meiotic spindle defects in mice by dietary and genetic strategies. Proc Natl Acad Sci U S A. 2011; 108(30):12319-24. PMC: 3145697. DOI: 10.1073/pnas.1018793108. View

18.

Pereira P, Dobreva M, Graham L, Huylebroeck D, Lawson K, Zwijsen A . Amnion formation in the mouse embryo: the single amniochorionic fold model. BMC Dev Biol. 2011; 11:48. PMC: 3163621. DOI: 10.1186/1471-213X-11-48. View

19.

Geraedts J, Montag M, Magli M, Repping S, Handyside A, Staessen C . Polar body array CGH for prediction of the status of the corresponding oocyte. Part I: clinical results. Hum Reprod. 2011; 26(11):3173-80. PMC: 3196878. DOI: 10.1093/humrep/der294. View

20.

Merriman J, Jennings P, McLaughlin E, Jones K . Effect of aging on superovulation efficiency, aneuploidy rates, and sister chromatid cohesion in mice aged up to 15 months. Biol Reprod. 2011; 86(2):49. DOI: 10.1095/biolreprod.111.095711. View