» Articles » PMID: 17114196

Influence of Follicular Fluid Meiosis-activating Sterol on Aneuploidy Rate and Precocious Chromatid Segregation in Aged Mouse Oocytes

Overview
Journal Hum Reprod
Date 2006 Nov 23
PMID 17114196
Citations 12
Authors
Affiliations
Soon will be listed here.
Abstract

Background: Follicular fluid meiosis-activating sterol (FF-MAS) protects young oocytes from precocious chromatid separation (predivision). Reduced expression of cohesion and checkpoint proteins and predivision has been hypothesized to occur in age-related aneuploidy in oocytes.

Methods: To know whether FF-MAS also protects aged oocytes from predivision and from age-related non-disjunction, we analysed chromosome constitution in mouse oocytes matured spontaneously with or without 10 microM FF-MAS and in hypoxanthine (HX)-arrested young and aged oocytes induced to resume maturation by FF-MAS. Messenger RNA for checkpoint protein MAD2 and cohesion protein SMC1beta was compared between oocytes matured with or without FF-MAS.

Results: Aged oocytes possessed many bivalents with single distal chiasma at meiosis I. Predivision was especially high in aged oocytes cultured sub-optimally to metaphase II in alpha-minimum essential medium (alpha-MEM). FF-MAS reduced predivision significantly (P < 0.001) but neither reduced non-disjunction nor induced aneuploidy in aged oocytes. Polyploidy was high in FF-MAS-stimulated maturation, in particular in the aged oocytes (P > 0.001). Relative levels of Smc1beta mRNA appeared increased by maturation in FF-MAS, and mitochondrial clustering was restored.

Conclusions: Sister chromatids of aged oocytes appear to be highly susceptible to precocious chromatid separation, especially when maturation is under sub-optimal conditions, e.g. in the absence of cumulus and FF-MAS. This may relate to some loss of chromatid cohesion during ageing. FF-MAS protects aged oocytes from predivision during maturation, possibly by supporting Smc1beta expression, thus reducing risks of meiotic errors, but it cannot prevent age-related non-disjunction. Aged oocytes appear prone to loss of co-ordination between nuclear maturation and cytokinesis suggesting age-related relaxed cell cycle control.

Citing Articles

Fat Mass and Obesity-Associated Protein Regulates Granulosa Cell Aging by Targeting Matrix Metalloproteinase-2 Gene Via an N6-Methyladenosine-YT521-B Homology Domain Family Member 2-Dependent Pathway in Aged Mice.

Li L, Yang L, Shen L, Zhao Y, Wang L, Zhang H Reprod Sci. 2024; 31(11):3498-3511.

PMID: 38995602 PMC: 11527923. DOI: 10.1007/s43032-024-01632-6.


Follicular fluid meiosis-activating sterol prevents porcine ovarian granulosa cells from hypoxia-induced apoptosis via inhibiting STAT4 expression.

Liu Z, Li C, Chen Q, Bai C, Wu G, Fu C J Anim Sci. 2024; 102.

PMID: 38713167 PMC: 11245709. DOI: 10.1093/jas/skae125.


Lipid Metabolic Process Involved in Oocyte Maturation During Folliculogenesis.

Liu T, Qu J, Tian M, Yang R, Song X, Li R Front Cell Dev Biol. 2022; 10:806890.

PMID: 35433675 PMC: 9009531. DOI: 10.3389/fcell.2022.806890.


Transcriptome-wide N6-methyladenine methylation in granulosa cells of women with decreased ovarian reserve.

Liu C, Li L, Yang B, Zhao Y, Dong X, Zhu L BMC Genomics. 2022; 23(1):240.

PMID: 35346019 PMC: 8961905. DOI: 10.1186/s12864-022-08462-3.


Advanced maternal age alters expression of maternal effect genes that are essential for human oocyte quality.

Zhang J, Liu X, Chen L, Zhang S, Zhang X, Hao C Aging (Albany NY). 2020; 12(4):3950-3961.

PMID: 32096767 PMC: 7066876. DOI: 10.18632/aging.102864.