Expression of Toll-Like Receptors 2 and 4 and Related Cytokines in Patients with Hepatic Cystic and Alveolar Echinococcosis

Overview

Journal Mediators Inflamm

Publisher Wiley

Specialties Biochemistry
Pathology

Date 2015 Dec 5

PMID 26635448

Citations 15

Authors

Tuerhongjiang Tuxun

Hai-Zhang Ma

Shadike Apaer

Heng Zhang

Amina Aierken

Yu-Peng Li

Ren-Yong Lin

Jin-Ming Zhao

Jin-Hui Zhang

Hao Wen

Affiliations

Soon will be listed here.

Abstract

Several studies have demonstrated the important role of Toll-like receptors in various parasitic infections. This study aims to explore expression of Toll-like receptors (TLRs) and related cytokines in patients with human cystic echinococcosis (CE) and alveolar echinococcosis (AE). 78 subjects including AE group (N = 28), CE group (N = 22), and healthy controls (HC, N = 28) were enrolled in this study. The mRNA expression levels of TLR2 and TLR4 in blood and hepatic tissue and plasma levels related cytokines were detected by using ELISA. Median levels of TLR2 mRNA in AE and CE groups were significantly elevated as compared with that in healthy control group. Median levels of TLR4 expression were increased in AE and CE. Plasma concentration levels of IL-5, IL-6, and IL-10 were slightly increased in AE and CE groups compared with those in HC group with no statistical differences (p > 0.05). The IL-23 concentration levels were significantly higher in AE and CE groups than that in HC subjects with statistical significance. The increased expression of TLR2 and IL-23 might play a potential role in modulating tissue infiltrative growth of the parasite and its persistence in the human host.

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References

Vuitton D . Echinococcosis and allergy. Clin Rev Allergy Immunol. 2004; 26(2):93-104. DOI: 10.1007/s12016-004-0004-2. View

Kawai T, Akira S . The role of pattern-recognition receptors in innate immunity: update on Toll-like receptors. Nat Immunol. 2010; 11(5):373-84. DOI: 10.1038/ni.1863. View

Floss D, Schroder J, Franke M, Scheller J . Insights into IL-23 biology: From structure to function. Cytokine Growth Factor Rev. 2015; 26(5):569-78. DOI: 10.1016/j.cytogfr.2015.07.005. View

Zhang W, Ross A, McManus D . Mechanisms of immunity in hydatid disease: implications for vaccine development. J Immunol. 2008; 181(10):6679-85. DOI: 10.4049/jimmunol.181.10.6679. View

Kern P, Wen H, Sato N, Vuitton D, Gruener B, Shao Y . WHO classification of alveolar echinococcosis: principles and application. Parasitol Int. 2005; 55 Suppl:S283-7. DOI: 10.1016/j.parint.2005.11.041. View

Kane C, Jung E, Pearce E . Schistosoma mansoni egg antigen-mediated modulation of Toll-like receptor (TLR)-induced activation occurs independently of TLR2, TLR4, and MyD88. Infect Immun. 2008; 76(12):5754-9. PMC: 2583582. DOI: 10.1128/IAI.00497-08. View

van Riet E, Everts B, Retra K, Phylipsen M, van Hellemond J, Tielens A . Combined TLR2 and TLR4 ligation in the context of bacterial or helminth extracts in human monocyte derived dendritic cells: molecular correlates for Th1/Th2 polarization. BMC Immunol. 2009; 10:9. PMC: 2672924. DOI: 10.1186/1471-2172-10-9. View

Carmena D, Cardona G . Echinococcosis in wild carnivorous species: epidemiology, genotypic diversity, and implications for veterinary public health. Vet Parasitol. 2014; 202(3-4):69-94. DOI: 10.1016/j.vetpar.2014.03.009. View

Smith P, Mangan N, Fallon P . Generation of parasite antigens for use in Toll-like receptor research. Methods Mol Biol. 2009; 517:401-13. DOI: 10.1007/978-1-59745-541-1_24. View

10.

Truchetet M, Beven L, Renaudin H, Douchet I, Ferandon C, Charron A . Potential role of Mycoplasma hominis in interleukin (IL)-17-producing CD4+ T-cell generation via induction of IL-23 secretion by human dendritic cells. J Infect Dis. 2011; 204(11):1796-805. DOI: 10.1093/infdis/jir630. View

11.

Lechner C, Gruner B, Huang X, Hoffmann W, Kern P, Soboslay P . Parasite-specific IL-17-type cytokine responses and soluble IL-17 receptor levels in Alveolar Echinococcosis patients. Clin Dev Immunol. 2012; 2012:735342. PMC: 3437316. DOI: 10.1155/2012/735342. View

12.

Ghosh D, Stumhofer J . Do you see what I see: Recognition of protozoan parasites by Toll-like receptors. Curr Immunol Rev. 2014; 9(3):129-140. PMC: 4223800. DOI: 10.2174/1573395509666131203225929. View

13.

McGovern K, Wilson E . Role of Chemokines and Trafficking of Immune Cells in Parasitic Infections. Curr Immunol Rev. 2014; 9(3):157-168. PMC: 4223871. DOI: 10.2174/1573395509666131217000000. View

14.

Chandra D, Naik S . Leishmania donovani infection down-regulates TLR2-stimulated IL-12p40 and activates IL-10 in cells of macrophage/monocytic lineage by modulating MAPK pathways through a contact-dependent mechanism. Clin Exp Immunol. 2008; 154(2):224-34. PMC: 2612710. DOI: 10.1111/j.1365-2249.2008.03741.x. View

15.

Gottstein B, Haag K, Walker M, Matsumoto J, Mejri N, Hemphill A . Molecular survival strategies of Echinococcus multilocularis in the murine host. Parasitol Int. 2005; 55 Suppl:S45-9. DOI: 10.1016/j.parint.2005.11.006. View

16.

Stojkovic M, Junghanss T . Cystic and alveolar echinococcosis. Handb Clin Neurol. 2013; 114:327-34. DOI: 10.1016/B978-0-444-53490-3.00026-1. View

17.

Medzhitov R, Preston-Hurlburt P, Janeway Jr C . A human homologue of the Drosophila Toll protein signals activation of adaptive immunity. Nature. 1997; 388(6640):394-7. DOI: 10.1038/41131. View

18.

Mourglia-Ettlin G, Marques J, Chabalgoity J, Dematteis S . Early peritoneal immune response during Echinococcus granulosus establishment displays a biphasic behavior. PLoS Negl Trop Dis. 2011; 5(8):e1293. PMC: 3166041. DOI: 10.1371/journal.pntd.0001293. View

19.

Trinchieri G, Sher A . Cooperation of Toll-like receptor signals in innate immune defence. Nat Rev Immunol. 2007; 7(3):179-90. DOI: 10.1038/nri2038. View

20.

Tuxun T, Wang J, Lin R, Shan J, Tai Q, Li T . Th17/Treg imbalance in patients with liver cystic echinococcosis. Parasite Immunol. 2012; 34(11):520-7. DOI: 10.1111/j.1365-3024.2012.01383.x. View