Correlation Between Proinflammatory Role of a Lectin from Typhonium Giganteum Engl. and Macrophage

Overview

Journal Int J Clin Exp Pathol

Specialty Pathology

Date 2015 Dec 1

PMID 26617695

Citations 1

Authors

Yaozong Pan

Hongli Yu

Hao Wu

Yeqing Chen

Kuilong Wang

Liping Liu

Yangping Jin

Chengchao Zhang

Affiliations

Soon will be listed here.

Abstract

Purpose: To analyze the correlation between proinflammatory effects of a lectin from Typhonium giganteum Engl. and macrophage.

Methods: T. giganteum lectin (TGL) was extracted from the tuber of T. giganteum and purified, and was then identified by using SDS-PAGE gel electrophoresis in combination with mass spectrometry. The morphologic changes of macrophage after being stimulated by TGL were observed with scanning electron microscopy. The influences of such stimulation on neutrophil migration were evaluated by establishing an in vitro macrophage-neutrophil co-culture migration model. By establishing a rat peritoneal macrophage in vitro cultured model, the effects of TGL stimulation on inflammatory factors TNF-α and IL-1β released by macrophage were analyzed. With p65 as the index, the expressions of the NF-κB signaling pathway in the cytoplasm and nucleus were detected before and after TGL stimulation respectively. Furthermore, we also investigated whether the inhibitor for NF-κB signaling pathway BAY11-7082 can block p65 nuclear translocation.

Results: After being stimulated by TGL, macrophage had increased volume, number of pseudopodia and gradually cracked cell membrane, accompanied by evidently induced migration of neutrophils due to released inflammatory factors. As the concentration of TGL varied, NF-κB's monomer p65 had different expression levels in the cytoplasm and nucleus, while BAY11-7082 can indeed block the nuclear translocation of p65.

Conclusions: TGL-induced inflammation was closely related to macrophage mediation.

Citing Articles

Utilizing ultrasound for the extraction of polysaccharides from the tuber of Typhonium giganteum Engl.: Extraction conditions, structural characterization and bioactivities.

Peng X, Wang J, Gao K, Wang Z, Deng Q, Wang Y Ultrason Sonochem. 2025; 113:107243.

PMID: 39864322 PMC: 11802366. DOI: 10.1016/j.ultsonch.2025.107243.

Typhonium giganteum Lectin Exerts A Pro-Inflammatory Effect on RAW 264.7 via ROS and The NF-κB Signaling Pathway.

Wang W, Wu H, Yu H, Zhang X, Cui G, Wang K Toxins (Basel). 2017; 9(9).

PMID: 28880234 PMC: 5618208. DOI: 10.3390/toxins9090275.

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