Interaction of RECQ4 and MCM10 is Important for Efficient DNA Replication Origin Firing in Human Cells

Overview

Journal Oncotarget

Specialty Oncology

Date 2015 Nov 21

PMID 26588054

Citations 20

Authors

Maciej Kliszczak

Hana Sedlackova

Ganesha P Pitchai

Werner W Streicher

Lumir Krejci

Ian D Hickson

Affiliations

Soon will be listed here.

Abstract

DNA replication is a highly coordinated process that is initiated at multiple replication origins in eukaryotes. These origins are bound by the origin recognition complex (ORC), which subsequently recruits the Mcm2-7 replicative helicase in a Cdt1/Cdc6-dependent manner. In budding yeast, two essential replication factors, Sld2 and Mcm10, are then important for the activation of replication origins. In humans, the putative Sld2 homolog, RECQ4, interacts with MCM10. Here, we have identified two mutants of human RECQ4 that are deficient in binding to MCM10. We show that these RECQ4 variants are able to complement the lethality of an avian cell RECQ4 deletion mutant, indicating that the essential function of RECQ4 in vertebrates is unlikely to require binding to MCM10. Nevertheless, we show that the RECQ4-MCM10 interaction is important for efficient replication origin firing.

Citing Articles

RECQ4-MUS81 interaction contributes to telomere maintenance with implications to Rothmund-Thomson syndrome.

Ashraf R, Polasek-Sedlackova H, Marini V, Prochazkova J, Hasanova Z, Zacpalova M Nat Commun. 2025; 16(1):1302.

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Multifaceted role of the DNA replication protein MCM10 in maintaining genome stability and its implication in human diseases.

Ahmed S, Sasikumar J, Laha S, Das S Cancer Metastasis Rev. 2024; 43(4):1353-1371.

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Quantity and quality of minichromosome maintenance protein complexes couple replication licensing to genome integrity.

Yadav A, Polasek-Sedlackova H Commun Biol. 2024; 7(1):167.

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Recognition and coacervation of G-quadruplexes by a multifunctional disordered region in RECQ4 helicase.

Papageorgiou A, Pospisilova M, Cibulka J, Ashraf R, Waudby C, Kaderavek P Nat Commun. 2023; 14(1):6751.

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The structural mechanism of dimeric DONSON in replicative helicase activation.

Cvetkovic M, Passaretti P, Butryn A, Reynolds-Winczura A, Kingsley G, Skagia A Mol Cell. 2023; 83(22):4017-4031.e9.

PMID: 37820732 PMC: 7616792. DOI: 10.1016/j.molcel.2023.09.029.

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